Article Resolution improvement of differential phase-contrast microscopy via tilt-series acquisition for environmental cell application

Kazutaka Mitsuishi SAMURAI ORCID (National Institute for Materials Science) ; Fumiaki Ichihashi ; Yoshio Takahashi ; Katsuaki Nakazawa SAMURAI ORCID (National Institute for Materials Science) ; Masaki Takeguchi SAMURAI ORCID (National Institute for Materials Science) ; Ayako Hashimoto SAMURAI ORCID (National Institute for Materials Science) ; Toshiaki Tanigaki

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Kazutaka Mitsuishi, Fumiaki Ichihashi, Yoshio Takahashi, Katsuaki Nakazawa, Masaki Takeguchi, Ayako Hashimoto, Toshiaki Tanigaki. Resolution improvement of differential phase-contrast microscopy via tilt-series acquisition for environmental cell application. Microscopy. 2025, 74 (2), 92-97. https://doi.org/10.1093/jmicro/dfae049

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(abstract)

A simple method that improves the resolution of the phase measurement of differential phase contrast (DPC) scanning transmission electron microscopy for closed-type environmental cell applications was developed and tested using a model sample simulating environmental cell observations. Because the top and bottom membranes of an environmental cell are typically far apart, the images from these embranes are shifted widely by tilt-series acquisition, and averaging the images after alignment can effectively eliminate undesired signals from the membranes while improving the signal from the object of interest. It was demonstrated that a phase precision of 2π/100 rad is well achievable using the proposed method for the sample in an environmental cell.

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Keyword: differential phase contrast, environmental cell, in situ transmission electron microscopy

Date published: 2025-03-31

Publisher: Oxford University Press (OUP)

Journal:

  • Microscopy (ISSN: 20505701) vol. 74 issue. 2 p. 92-97

Funding:

  • Innovative Science and Technology Initiative for Security JPJ004596
  • Innovative Science and Technology Initiative for Security JPJ004596

Manuscript type: Author's version (Accepted manuscript)

MDR DOI: https://doi.org/10.48505/nims.5884

First published URL: https://doi.org/10.1093/jmicro/dfae049

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Updated at: 2025-11-11 08:30:20 +0900

Published on MDR: 2025-11-11 08:22:14 +0900

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