# Quantifying the Thresholds of the Phospholipid Surface Density for Nonspecific Protein Adsorption and Desorption at the Triacylglycerol/Water Interface

https://mdr.nims.go.jp/datasets/adaa8eef-ed82-4b0d-8d8e-abda66bee80b

## File

- [Supporting_information_250901.pdf](https://mdr.nims.go.jp/filesets/6f2bc510-7f14-4b7b-aa8a-2719bffe1abb/download) ([Detail](https://mdr.nims.go.jp/filesets/6f2bc510-7f14-4b7b-aa8a-2719bffe1abb.md))

## Id

adaa8eef-ed82-4b0d-8d8e-abda66bee80b

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-09-24T00:20:10.670451Z

## Updated at

2025-09-24T07:30:26.495521Z

## Published at

2025-09-24T07:19:13.622745Z

## Doi

https://doi.org/10.48505/nims.5776

## First published url

https://doi.org/10.1021/acs.langmuir.5c03216

## Date published

2025-09-23

## Recorded date published

2025-9-23

## Resource type

dataset

## Manuscript type

na

## Collection



## Title

- title: Quantifying the Thresholds of the Phospholipid Surface Density for Nonspecific
    Protein Adsorption and Desorption at the Triacylglycerol/Water Interface
  title_type: original
  lang: en

## Description

- description: Quantitative investigation of protein adsorption and desorption at
    triacylglycerol/water interfaces is of great importance for understanding the
    properties and functions of intracellular lipid droplets. In this study, we investigated
    the adsorption and desorption of cytochrome c, lysozyme, and bovine serum albumin
    at the tricaprylin/water interface covered with 1,2-dioleoyl-sn-glycero-3-phosphocholine
    (DOPC) using a surface pressure measurement system and a pendant drop tensiometer.
    The aim of this study was to understand the quantitative relationships between
    the area per DOPC molecule and the protein adsorption and desorption. We found
    that the area per DOPC molecule required to preclude bovine serum albumin adsorption
    was much smaller than that required to preclude the adsorption of cytochrome c
    or lysozyme. However, the area per DOPC molecule required for all of the bound
    protein molecules to desorb from the interface was independent of the type of
    protein, and this threshold area per DOPC molecule was in good agreement with
    an area per lipid value previously reported for fully hydrated DOPC bilayers.
  description_type: abstract
  lang: und

## Creator

- name: Chiho Kataoka-Hamai
  role: author
  orcid: https://orcid.org/0000-0002-4068-0405

## Contact agent



## Publisher

organization: American Chemical Society (ACS)

## Managing organization



## Keyword

- subject: Lipid droplets
  schema: not_defined
- subject: triacylglycerol
  schema: not_defined
- subject: oil/water interface
  schema: not_defined
- subject: protein
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Langmuir
  issn: '07437463'
  volume: '41'
  issue: '37'
  start_page: 25431
  end_page: 25438

## Conference



## Related item

- title: Quantifying the Thresholds of the Phospholipid Surface Density for Nonspecific
    Protein Adsorption and Desorption at the Triacylglycerol/Water Interface
  identifier: https://doi.org/10.1021/acs.langmuir.5c03216
  identifier_type: DOI
  relation_type: is_supplement_to
  related_item_type: journal_article

## Funding

- identifier: 22K05179
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 6f2bc510-7f14-4b7b-aa8a-2719bffe1abb
  filename: Supporting_information_250901.pdf
  content_type: application/pdf
  size: 496983
  md5: bfa9e206af4140ce3bdc4cbaa3ca98d8

## Thumbnail

fileset_id: 6f2bc510-7f14-4b7b-aa8a-2719bffe1abb
filename: Supporting_information_250901.pdf