# Long-term exposure in high viscous microenvironment facilitates acquired drug resistance of colon cancer cells to doxorubicin

https://mdr.nims.go.jp/datasets/ac151f32-0514-4a53-b0ac-51ced3b5a6fb

## File

- [EJPB-D-25-00777.pdf](https://mdr.nims.go.jp/filesets/9390122a-b53f-4940-a0ae-5115f2265490/download) ([Detail](https://mdr.nims.go.jp/filesets/9390122a-b53f-4940-a0ae-5115f2265490.md))

## Id

ac151f32-0514-4a53-b0ac-51ced3b5a6fb

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-10-13T00:25:56.750037Z

## Updated at

2025-10-14T01:43:53.740622Z

## Published at

2026-09-01T23:25:16.583835Z

## Doi

https://doi.org/10.48505/nims.5805

## First published url

https://doi.org/10.1016/j.ejpb.2025.114852

## Date published

2025-09-02

## Recorded date published

2025-11

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Long-term exposure in high viscous microenvironment facilitates acquired
    drug resistance of colon cancer cells to doxorubicin
  title_type: original
  lang: en

## Description

- description: In this study, the effect of microenvironmental viscosity on ADR of
    colon cancer cells to doxorubicin (DOX) was investigated by long-term culture
    of colon cancer cells in different viscous media supplemented with DOX. The 50%
    inhibitory concentration (IC50) value of drug-resistant cells established by culturing
    in high viscosity media was significantly higher than that of cells cultured in
    low viscosity media. The drug-resistant colon cancer cells established in high
    viscosity media exhibited higher expression levels of drug resistance-related
    genes (ABCC2 and ABCG2), higher migration ability and lower proliferation ability
    than the cells established in low viscosity medium. Long-term exposure in DOX-containing
    high viscosity media made the cells more tolerant to DOX and facilitated the ADR
    of colon cancer cells to DOX.
  description_type: abstract
  lang: und

## Creator

- name: Tianjiao Zeng
  role: author
  orcid: https://orcid.org/0000-0002-1286-0337
- name: Chengyu Lu
  role: author
- name: Man Wang
  role: author
- name: Huajian Chen
  role: author
- name: Toru Yoshitomi
  role: author
  orcid: https://orcid.org/0000-0003-3847-1812
- name: Naoki Kawazoe
  role: author
  orcid: https://orcid.org/0000-0003-3916-0709
- name: Yingnan Yang
  role: author
- name: Guoping Chen
  role: author
  orcid: https://orcid.org/0000-0001-6753-3678

## Contact agent



## Publisher

organization: Elsevier BV

## Managing organization



## Keyword

- subject: high viscous microenvironment
  schema: not_defined
- subject: drug resistance
  schema: not_defined
- subject: colon cancer cell
  schema: not_defined
- subject: doxorubicin
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2025-09-02
end_date: 2026-09-02

## Journal

- title: European Journal of Pharmaceutics and Biopharmaceutics
  issn: '09396411'
  volume: '216'
  article_number: '114852'

## Conference



## Related item



## Funding

- funder_name: Foundation for Promotion of Material Science and Technology of Japan
- identifier: 24K03289
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 9390122a-b53f-4940-a0ae-5115f2265490
  filename: EJPB-D-25-00777.pdf
  content_type: application/pdf
  size: 3147813
  md5: 1eaa9b38cc41483dab4ed54ad45a8df1

## Thumbnail

fileset_id: 9390122a-b53f-4940-a0ae-5115f2265490
filename: EJPB-D-25-00777.pdf