# Rapid bone formation and remodeling via vascular infiltration of a hydroxyapatite/collagen nanocomposite beneath the calvarial periosteum

https://mdr.nims.go.jp/datasets/a5cdefbd-f5b9-4059-b281-3e68cec550cd

## Files

- [1-s2.0-S2666534426000048-main.pdf](https://mdr.nims.go.jp/filesets/6438114f-0794-477b-8b66-9b25c340bd70/download) ([Detail](https://mdr.nims.go.jp/filesets/6438114f-0794-477b-8b66-9b25c340bd70.md))

## Id

a5cdefbd-f5b9-4059-b281-3e68cec550cd

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2026-02-20T06:04:36.168076Z

## Updated at

2026-02-21T07:30:04.694666Z

## Published at

2026-02-21T04:38:39.573548Z

## Doi



## First published url

https://doi.org/10.1016/j.bbiosy.2026.100130

## Date published

2026-01-22

## Recorded date published

2026-3

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Rapid bone formation and remodeling via vascular infiltration of a hydroxyapatite/collagen
    nanocomposite beneath the calvarial periosteum
  title_type: original
  lang: en

## Description

- description: "This study investigated the time-dependent osteogenic potential of
    an organic-inorganic composite bone filler (hydroxyapatite/collagen bone-like
    nanocomposite, HAp/Col) using a rat calvarial model, assessed by immunohistochemistry
    and bone histomorphometry.\r\nBy day 3, vascular endothelial growth factor receptor
    2 (VEGFR2)-positive cells were detected on the HAp/Col surface, indicating early
    vascular invasion. By day 5, vascular infiltration had extended into the scaffold,
    where double staining with tartrate resistant acid phosphatase (TRAP)/ alkaline
    phosphatase (ALP) revealed osteoclast-mediated material resorption and osteoblast-mediated
    bone matrix deposition around vascular cavities. By day 7, bone remodeling within
    HAp/Col was markedly active, with clusters of osteoblasts producing new bone matrix.
    Outside the scaffold, osteoblasts adhered to its surface and elongated, enhancing
    bone formation through the space-forming effect of HAp/Col swelling.\r\nFurthermore,
    collagen triple helix repeat-containing protein 1 (CTHRC1) expression increased
    progressively from days 3 to 7, as confirmed by immunostaining and qRT-PCR, suggesting
    its role as a coupling factor between osteoclast activity and osteoblast differentiation.\r\nThese
    findings suggest that HAp/Col is associated with cellular activities related to
    bone remodeling, including vascular invasion and osteoclast/osteoblast recruitment.
    HAp/Col demonstrates biocompatibility and in vivo tissue compatibility as a candidate
    biomaterial for medical and dental applications."
  description_type: abstract
  lang: und

## Creator

- name: Hanae Arai
  role: author
- name: Masayoshi Uezono
  role: author
  orcid: https://orcid.org/0000-0003-0976-7788
- name: Masanori Kikuchi
  role: author
  orcid: https://orcid.org/0000-0002-9451-8147
- name: Hitoshi Amano
  role: author
- name: Chen Derong
  role: author
  orcid: https://orcid.org/0009-0006-3371-0086
- name: Kazuhiro Aoki
  role: author
  orcid: https://orcid.org/0000-0001-6643-6315
- name: Keiji Moriyama
  role: author

## Contact agent



## Publisher

organization: Elsevier BV

## Managing organization



## Keyword

- subject: Hydroxyapatite/collagen bone-like nanocomposite
  schema: not_defined
- subject: Bone remodeling
  schema: not_defined
- subject: Bone replacement
  schema: not_defined
- subject: Osteoblast osteoclast angiogenesis
  schema: not_defined
- subject: VEGF2R
  schema: not_defined
- subject: CTHRC1
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/

## Other identifier(s)



## Data origin



## Embargo



## Journal

- title: Biomaterials and Biosystems
  issn: '26665344'
  volume: '21'
  article_number: '100130'

## Conference



## Related item



## Funding

- funder_name: Ministry of Education, Culture, Sports, Science and Technology
  description: the establishment of university fellowship toward the creation of science
    technology innovation
- identifier: 21K17177a
  funder_name: Japan Society for the Promotion of Science (JSPS)
  description: KAKENHI Grant

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 6438114f-0794-477b-8b66-9b25c340bd70
  filename: 1-s2.0-S2666534426000048-main.pdf
  content_type: application/pdf
  size: 16069635
  md5: 6fcd637b4c617717f484158049cb3875

## Thumbnail

fileset_id: 6438114f-0794-477b-8b66-9b25c340bd70
filename: 1-s2.0-S2666534426000048-main.pdf