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Our body is formed through self-assembly. Sophisticated and hierarchical structures of our tissues and organs are built from individual smaller components such as DNA, peptides, collagens and cells. Inspired by nature’s design principles, we have developed a cellular flocculation system using cellulose nanofibers modified with concentrated polymer brushes (CNF-CPBs) as artificial ECMs. First, we demonstrated that human hepatocyte cells (HepG2) spontaneously formed flocs (self-assembled) with negatively charged CNF-CPBs, affording to able to control their sizes and shapes, and it enhanced the cell functions. Next, by varying the charge of polymers (zeta-potential = -23, -19, 17, 11 mV), we revealed that appropriate electrostatic interactions are essential for floc formation and the regulation of cell functions. Importantly, the results indicate that colloidal flocculation theory is the driving mechanism behind this unique phenomenon. Furthermore, CNF-CPBs with different fiber lengths (0.16 ~ 1.8 μm) were used for the cellular flocculation (HepG2, L929 and hTERT). We confirmed that the size of the flocs is greatly affected by the concentration and length of CNF-CPB. Finally, considering the potential of CNF-CPBs as a new 3d cell culture tool, particularly as a novel cellular self-assemble system, we examined cartilage regeneration of human mesenchymal stem cells (hMSCs) using CNF-CPBs. Interestingly, hMSCs were self-assembled with CNF-CPBs and formed ball- or sheet-type giant cellular structures, depending on the CNF-CPB concentration (0.1, 0.05, 0.005 wt%) in the presence of chondrogenic induction medium (Fig. 1). The highest concentration of CNF-CPB remarkably enhanced chondrogenic differentiation, in comparison to lower concentrations and a cell pellet. The details will be discussed.

権利情報:

• In Copyright
  

キーワード: Concentrated polymer brush, Cellulose nanofiber, Flocculation, Cell, Tissue engineering

会議: ISF2024 (International Symposium on Fiber Science and Technology 2024) (2024-11-25 - 2024-11-28)

研究助成金:

• JSPS 22H02133

原稿種別: 論文以外のデータ

MDR DOI: https://doi.org/10.48505/nims.5861

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更新時刻: 2025-11-06 12:30:47 +0900

MDRでの公開時刻: 2025-11-06 12:24:52 +0900