# Molecular design of phosphatidylserine-inspired polymers for efficient anti-inflammatory therapy via enhanced interaction with Tim-4

https://mdr.nims.go.jp/datasets/a09ab1cb-c55b-4444-966c-4a285f5bdf0f

## File

- [26_PolymerJ.pdf](https://mdr.nims.go.jp/filesets/41fa37f5-785d-41fe-be96-6e913c7dae3f/download) ([Detail](https://mdr.nims.go.jp/filesets/41fa37f5-785d-41fe-be96-6e913c7dae3f.md))

## Id

a09ab1cb-c55b-4444-966c-4a285f5bdf0f

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2026-04-15T23:54:00.943139Z

## Updated at

2026-04-16T06:12:41.991886Z

## Published at

2026-04-16T07:26:11.575571Z

## Doi



## First published url

https://doi.org/10.1038/s41428-025-01140-7

## Date published

2026-01-09

## Recorded date published

2026-4

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Molecular design of phosphatidylserine-inspired polymers for efficient anti-inflammatory
    therapy via enhanced interaction with Tim-4
  title_type: original
  lang: en

## Description

- description: This study investigated the interaction between phosphatidylserine
    (PS)-inspired polymers　and T cell immunoglobulin and mucin-like domain-containing
    protein 4 (Tim-4) by systematically varying the monomer structure and copolymer
    composition. A series of alkyl-substituted PS-inspired monomers was synthesized
    using a modified phosphoramidite method, and well-defined homopolymers and 2-hydroxyethyl
    methacrylate (HEMA)-containing copolymers were prepared via reversible addition–fragmentation
    chain-transfer polymerization. Structural analyses using 1H nuclear magnetic resonance
    and gel permeation chromatography confirmed the successful synthesis with controlled
    molecular weights. Biolayer interferometry was used to quantify Tim-4 binding,
    revealing a non-monotonic effect of alkyl substitution, whereas the incorporation
    of HEMA consistently enhanced Tim-4 binding in a composition-dependent manner.
    Biological evaluation using RAW-Blue macrophages showed that the homopolymers
    did not significantly affect interleukin-6 (IL-6) secretion, whereas the copolymers
    selectively suppressed IL-6 production. Notably, the copolymer containing 50 mol%
    PS units exhibited the strongest IL-6 suppression, and the HEMA-containing copolymers
    exhibited anti-inflammatory activity even at lower PS concentrations than the
    homopolymers. These results demonstrate that copolymer composition critically
    influences receptor interactions and immune modulation. This study highlights
    the potential of PS-inspired copolymers as biomaterials that mimic apoptotic cell
    signals and exert efficient anti-inflammatory effects through an optimized molecular
    design.
  description_type: abstract
  lang: und

## Creator

- name: Kosuke Sato
  role: author
  organization: National Institute for Materials Science
- name: Ahmed Nabil
  role: author
- name: Komol Kanta Sharker
  role: author
- name: Kouichi Shiraishi
  role: author
- name: Mitsuhiro Ebara
  role: author
  orcid: https://orcid.org/0000-0002-7906-0350
  organization: National Institute for Materials Science

## Contact agent



## Publisher

organization: Springer Science and Business Media LLC

## Managing organization



## Keyword

- subject: phosphatidylserine
  schema: not_defined
- subject: anti-inflammatory
  schema: not_defined
- subject: Tim-4
  schema: not_defined
- subject: MPS
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/
  date_licensed: 2026-01-09

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## Journal

- title: Polymer Journal
  issn: '13490540'
  start_page: 407
  end_page: 415

## Conference



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## Funding

- identifier: JP19H04476
  funder_name: MEXT | Japan Society for the Promotion of Science
- identifier: JP20H05877
  funder_name: MEXT | Japan Society for the Promotion of Science
- identifier: 24KJ0230
  funder_name: MEXT | Japan Society for the Promotion of Science
- identifier: 20H0587
  funder_name: MEXT | Japan Society for the Promotion of Science
- identifier: 23K28436
  funder_name: MEXT | Japan Society for the Promotion of Science

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## Fileset

- id: 41fa37f5-785d-41fe-be96-6e913c7dae3f
  filename: 26_PolymerJ.pdf
  content_type: application/pdf
  size: 1246401
  md5: f20500eddecde4faf81c3b4865499b9a

## Thumbnail

fileset_id: 41fa37f5-785d-41fe-be96-6e913c7dae3f
filename: 26_PolymerJ.pdf