# CD44‐Binding Peptide‐Functionalized Antibiofouling Polymer Surface for High‐Performance Separation of Human Mesenchymal Stromal Cells

https://mdr.nims.go.jp/datasets/9f482ab0-2acb-4c50-8b4e-949b6aa3102d

## File

- [ChemBioChem - 2026 - Kato - CD44‐Binding Peptide‐Functionalized Antibiofouling Polymer Surface for High‐Performance (1).pdf](https://mdr.nims.go.jp/filesets/7dc284a2-4e2f-45a9-853b-1ccfa1bdd6fe/download) ([Detail](https://mdr.nims.go.jp/filesets/7dc284a2-4e2f-45a9-853b-1ccfa1bdd6fe.md))

## Id

9f482ab0-2acb-4c50-8b4e-949b6aa3102d

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2026-02-26T01:13:19.871995Z

## Updated at

2026-02-26T23:30:32.980971Z

## Published at

2026-02-26T08:51:09.544443Z

## Doi



## First published url

https://doi.org/10.1002/cbic.202500822

## Date published

2026-02-12

## Recorded date published

2026-2-12

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: CD44‐Binding Peptide‐Functionalized Antibiofouling Polymer Surface for High‐Performance
    Separation of Human Mesenchymal Stromal Cells
  title_type: original
  lang: en

## Description

- description: Cell transplantation therapy represents a promising strategy in next-generation
    regenerative medicine; however, its clinical application remains limited by the
    absence of safe and label-free methods for obtaining pure populations of functional
    cells. This study reports a peptide-functionalized zwitterionic polymer system
    designed for the selective and non-invasive isolation of human mesenchymal stem
    cells (hMSCs) from heterogeneous cell populations. The substrate was modified
    with poly(CMBMAm-co-PGMAn-co-MPTMS1) (PCmPnM1), synthesized via free radical polymerization
    and subsequently conjugated with a CD44-binding peptide (CD44BP). Among the compositions
    examined, the PC7P2M1–CD44BP surface exhibited superior antifouling properties,
    effectively suppressing nonspecific protein adsorption and adhesion of non-target
    cells while selectively capturing hMSCs. A column packed with PC7P2M1–CD44BP–modified
    silica microparticles successfully isolated high-purity hMSCs from mixed cell
    suspensions within 35 minutes. Flow cytometry confirmed that the later-eluted
    cells exhibited higher CD44 and CD105 expression, indicating separation based
    on antigen expression. The isolated hMSCs maintained proliferation and differentiation
    capacities equivalent to those of pre-separated cells, demonstrating that this
    device preserves cell functionality. This peptide–polymer hybrid column provides
    a simple and clinically adaptable platform for safe, label-free purification of
    hMSCs intended for cell transplantation therapy.
  description_type: abstract
  lang: und

## Creator

- name: Moe Kato
  role: author
- name: Tadashi Nakaji
  role: author
- name: Kazuaki Matsumura
  role: author
- name: Chiaki Yoshikawa
  role: author
  orcid: https://orcid.org/0000-0002-6589-387X
  organization: National Institute for Materials Science
- name: Yuki Usui
  role: author
- name: Takahiro Kishioka
  role: author
- name: Taito Nishino
  role: author

## Contact agent



## Publisher

organization: Wiley

## Managing organization



## Keyword

- subject: antifouling
  schema: not_defined
- subject: cell separation
  schema: not_defined
- subject: hMSC
  schema: not_defined
- subject: label-free purification
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/
  date_licensed: 2026-02-12

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: CHEMBIOCHEM
  issn: '14394227'
  volume: '27'
  issue: '3'

## Conference



## Related item



## Funding

- funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 7dc284a2-4e2f-45a9-853b-1ccfa1bdd6fe
  filename: ChemBioChem - 2026 - Kato - CD44‐Binding Peptide‐Functionalized Antibiofouling
    Polymer Surface for High‐Performance (1).pdf
  content_type: application/pdf
  size: 1927592
  md5: d5b1719fbcd5bac6a63f8aefbecc1727

## Thumbnail

fileset_id: 7dc284a2-4e2f-45a9-853b-1ccfa1bdd6fe
filename: ChemBioChem - 2026 - Kato - CD44‐Binding Peptide‐Functionalized Antibiofouling
  Polymer Surface for High‐Performance (1).pdf