# Effects of G-Quadruplex Ligands on the Topology, Stability, and Immunostimulatory Properties of G-Quadruplex-Based CpG Oligodeoxynucleotides

https://mdr.nims.go.jp/datasets/96b8ab45-cb66-4c85-a148-5a1af9ba2628

## Files

- [著者最終稿.pdf](https://mdr.nims.go.jp/filesets/0b78002f-1979-4c79-8888-5c0871f44641/download) ([Detail](https://mdr.nims.go.jp/filesets/0b78002f-1979-4c79-8888-5c0871f44641.md))
- [著者最終稿_Supporting information.pdf](https://mdr.nims.go.jp/filesets/cd59c853-e8a0-4dee-ba45-82a7be96db9f/download) ([Detail](https://mdr.nims.go.jp/filesets/cd59c853-e8a0-4dee-ba45-82a7be96db9f.md))

## Id

96b8ab45-cb66-4c85-a148-5a1af9ba2628

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-10-03T00:01:16.896625Z

## Updated at

2024-10-03T07:30:22.006070Z

## Published at

2024-10-03T07:30:22.277509Z

## Doi

https://doi.org/10.48505/nims.4796

## First published url

https://doi.org/10.1021/acschembio.1c00904

## Date published

2022-07-15

## Recorded date published

2022-7-15

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Effects of G-Quadruplex Ligands on the Topology, Stability, and Immunostimulatory
    Properties of G-Quadruplex-Based CpG Oligodeoxynucleotides
  title_type: original
  lang: en

## Description

- description: G4 ligands, which are small molecules that bind to G4 ODNs with high
    affinity, were reported to improve the stability of G4. In this study, we propose
    to increase the stability and function of G4 CpG ODNs using G4 ligands. We show
    the effects of two G4 ligands, named L2H2-6OTD (L2H2) and L2G2-2M2EG-6OTD (L2G2),
    on the topology, stability, and immunostimulatory properties of a monomeric hybrid-type
    G4 CpG ODN containing CpG motifs in the central loop, named GD3. We found that
    L2H2 helps maintain the hybrid G4 topology of GD3 whereas L2G2 induces parallel
    G4 formation. Both G4 ligands increase the thermodynamic and nuclease stability
    of GD3. However, only GD3 associated with L2H2 binds efficiently to TLR9 and evokes
    a higher immune response from mouse macrophage-like RAW264 cells. GD3 associated
    with L2G2 does not bind efficiently to TLR9 and elicits lower cytokine production.
    Our results demonstrate that the potential to enhance immunostimulatory properties
    depends on the ability of G4 ligands to maintain and stabilize the hybrid G4 of
    GD3. We anticipate that our findings will facilitate the development of more effective
    G4 CpG ODN-based vaccine adjuvants against infectious diseases.
  description_type: abstract
  lang: und

## Creator

- name: Anh Thi Tram Tu
  role: author
- name: Kazuaki Hoshi
  role: author
  organization: National Institute for Materials Science
- name: Yue Ma
  role: author
- name: Taiji Oyama
  role: author
- name: Satoko Suzuki
  role: author
- name: Kaori Tsukakoshi
  role: author
- name: Kazuo Nagasawa
  role: author
- name: Kazunori Ikebukuro
  role: author
- name: Tomohiko Yamazaki
  role: author
  orcid: https://orcid.org/0000-0003-2136-8042
  organization: National Institute for Materials Science

## Contact agent



## Publisher

organization: American Chemical Society (ACS)

## Managing organization



## Keyword

- subject: guanine quadruplex
  schema: not_defined
- subject: CpG oligodeoxynucleotide
  schema: not_defined
- subject: toll-like receptor 9
  schema: not_defined
- subject: phosphodiester backbone
  schema: not_defined
- subject: G-quadruplex ligand
  schema: not_defined

## Rights

- description: This document is the Accepted Manuscript version of a Published Work
    that appeared in final form in ACS Chemical Biology, copyright © 2022 American
    Chemical Society after peer review and technical editing by the publisher. To
    access the final edited and published work see https://doi.org/10.1021/acschembio.1c00904
  identifier: http://rightsstatements.org/vocab/InC/1.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2022-06-29
end_date: 2023-06-29

## Journal

- title: ACS Chemical Biology
  issn: '15548929'
  volume: '17'
  issue: '7'
  start_page: 1703
  end_page: 1713

## Conference



## Related item



## Funding

- funder_name: Ministry of Education, Culture, Sports, Science and Technology
- identifier: 17KK0122
  funder_name: Japan Society for the Promotion of Science
- identifier: 18K04858
  funder_name: Japan Society for the Promotion of Science
- identifier: 21K19057
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 0b78002f-1979-4c79-8888-5c0871f44641
  filename: 著者最終稿.pdf
  content_type: application/pdf
  size: 931935
  md5: 9eff397b6709168d00534413a7d018d1
- id: cd59c853-e8a0-4dee-ba45-82a7be96db9f
  filename: 著者最終稿_Supporting information.pdf
  content_type: application/pdf
  size: 552616
  md5: 3e8215a8413be1fe607623870131aec3

## Thumbnail

fileset_id: 0b78002f-1979-4c79-8888-5c0871f44641
filename: 著者最終稿.pdf