# Microtubules Disruption Alters the Cellular Structures and Mechanics Depending on Underlying Chemical Cues

https://mdr.nims.go.jp/datasets/95fb1cc2-3fdd-404c-b534-66c2d5cb109a

## File

- [Small - 2024 - Abdellatef - Microtubules Disru.pdf](https://mdr.nims.go.jp/filesets/cb7ccca4-490a-40ab-b0db-f24ed8519894/download) ([Detail](https://mdr.nims.go.jp/filesets/cb7ccca4-490a-40ab-b0db-f24ed8519894.md))

## Id

95fb1cc2-3fdd-404c-b534-66c2d5cb109a

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-06-09T22:50:04.965800Z

## Updated at

2025-06-10T03:30:28.021807Z

## Published at

2025-06-10T03:22:43.278940Z

## Doi



## First published url

https://doi.org/10.1002/smll.202312282

## Date published

2024-09-29

## Recorded date published

2025-4

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Microtubules Disruption Alters the Cellular Structures and Mechanics Depending
    on Underlying Chemical Cues
  title_type: original
  lang: en

## Description

- description: The extracellular matrix determines cell morphology and stiffness by
    manipulating the cytoskeleton. The impacts of extracellular matrix cues, including
    the mechanical and topographical cues on microtubules and their role in biological
    behaviors, are previously studied. However, there is a lack of understanding about
    how microtubules (MTs) are affected by environmental chemical cues, such as extracellular
    matrix density. Specifically, it is crucial to understand the connection between
    cellular morphology and mechanics induced by chemical cues and the role of microtubules
    in these cellular responses. To address this, surfaces with high and low cRGD
    (cyclic Arginine-Glycine-Aspartic acid) peptide ligand densities are used. The
    cRGD is diluted with a bioinert ligand to prevent surface native cellular remodeling.
    The cellular morphology, actin, and microtubules differ on these surfaces. Confocal
    fluorescence microscopes and atomic force microscopy (AFM) are used to determine
    the structural and mechanical cellular responses with and without microtubules.
    Microtubules are vital as an intracellular scaffold in elongated morphology correlated
    with low cRGD compared to rounded morphology in high cRGD substrates. The contributions
    of MTs to nucleus morphology and cellular mechanics are based on the underlying
    cRGD densities. Finally, this study reveals a significant correlation between
    MTs, actin networks, and vimentin in response to the underlying densities of cRGD.
  description_type: abstract
  lang: und

## Creator

- name: Shimaa A. Abdellatef
  role: author
- name: Hongxin Wang
  role: author
- name: Jun Nakanishi
  role: author
  orcid: https://orcid.org/0000-0003-4457-6581

## Contact agent



## Publisher

organization: Wiley

## Managing organization



## Keyword

- subject: Mechanobiology
  schema: not_defined
- subject: Tensegrity
  schema: not_defined
- subject: Microtubules
  schema: not_defined
- subject: cRGD
  schema: not_defined
- subject: Cytoekeleton
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin



## Embargo



## Journal

- title: Small
  issn: '16136810'
  volume: '21'
  issue: '13'

## Conference



## Related item



## Funding

- identifier: 22H00596
  funder_name: Japan Society for the Promotion of Science
- identifier: 21J40229
  funder_name: Japan Society for the Promotion of Science
- identifier: 23KJ2167
  funder_name: Japan Society for the Promotion of Science
- identifier: 23K17481
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Custom property



## Fileset

- id: cb7ccca4-490a-40ab-b0db-f24ed8519894
  filename: Small - 2024 - Abdellatef - Microtubules Disru.pdf
  content_type: application/pdf
  size: 2656911
  md5: f9bdc95ab426bb6c0c673a3d09e3d727

## Thumbnail

fileset_id: cb7ccca4-490a-40ab-b0db-f24ed8519894
filename: Small - 2024 - Abdellatef - Microtubules Disru.pdf