# Lysosomal enzymes and the oxygen burst capability of monocyte-derived macrophages in active drug-resistant tuberculosis patients in relation to cell attachment

https://mdr.nims.go.jp/datasets/8ed741f3-a1c2-496b-9d5a-2fb292150a2e

## File

- [Lysosomal enzymes and the oxygen-2024-Iswanti.pdf](https://mdr.nims.go.jp/filesets/abd3be10-780e-4da3-bb54-e16cc1426ac8/download) ([Detail](https://mdr.nims.go.jp/filesets/abd3be10-780e-4da3-bb54-e16cc1426ac8.md))

## Id

8ed741f3-a1c2-496b-9d5a-2fb292150a2e

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-10-15T23:41:19.322507Z

## Updated at

2024-12-10T07:55:31.718521Z

## Published at

2024-12-10T07:55:31.785089Z

## Doi



## First published url

https://doi.org/10.1016/j.tube.2024.102498

## Date published

2024-02-24

## Recorded date published

2024-5

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Lysosomal enzymes and the oxygen burst capability of monocyte-derived macrophages
    in active drug-resistant tuberculosis patients in relation to cell attachment
  title_type: original
  lang: en

## Description

- description: Drug resistance to tuberculosis (TB) has become an obstacle in eliminating
    tuberculosis. The transmission of drug-resistant TB from patients increases the
    incidence of primary drug-resistant (DR) TB in individuals who are in close contact.
    Therefore, it is necessary to incorporate an immunological approach into preventive
    therapy. This study focuses on the activity of lysosomal enzymes, oxygen bursts,
    and the attachment ability of macrophages among individuals diagnosed with active
    drug-resistant TB compared with close contacts with latent TB or healthy cases.
    We measured macrophage oxygen burst ability (Water-soluble tetrazolium salt (WST)
    test, Nitric Oxide production, and myeloperoxidase activity) and the degradative
    ability of lysosomes (activity of the beta-glucuronidase and acid phosphatase
    enzymes). Six active DR-TB patients and 18 close-contact cases (8 Latent Tuberculosis
    Infection (LTBI); 10 healthy) were recruited at Universitas Indonesia Hospital.
    The macrophage attachment of the LTBI group was higher than in the other groups.
    NO production, myeloperoxidase activity, beta-glucuronidase, and acid phosphatase
    were higher in the active DR-TB group. A negative correlation was uncovered between
    phagocytosis and NO production, myeloperoxidase activity, and lysosomal enzymes.
    The difference in macrophage function is expected to be a further reference in
    active DR-TB treatment or preventive therapy.
  description_type: abstract
  lang: und

## Creator

- name: Febriana Catur Iswanti
  role: author
- name: Kurnia Maidarmi Handayani
  role: author
- name: Ardiana Kusumaningrum
  role: author
- name: Tomohiko Yamazaki
  role: author
  orcid: https://orcid.org/0000-0003-2136-8042
  organization: National Institute for Materials Science
- name: Diah Handayani
  role: author
- name: Mohamad Sadikin
  role: author

## Contact agent



## Publisher

organization: Elsevier BV

## Managing organization



## Keyword

- subject: Humans
  schema: not_defined
- subject: Mycobacterium tuberculosis
  schema: not_defined
- subject: Tuberculosis/diagnosis
  schema: not_defined
- subject: Latent Tuberculosis
  schema: not_defined
- subject: Tuberculosis, Multidrug-Resistant/drug therapy
  schema: not_defined
- subject: Macrophages
  schema: not_defined
- subject: Glucuronidase
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Tuberculosis
  issn: '14729792'
  volume: '146'
  article_number: '102498'

## Conference



## Related item



## Funding

- funder_name: University of Indonesia Directorate of Research and Development

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: abd3be10-780e-4da3-bb54-e16cc1426ac8
  filename: Lysosomal enzymes and the oxygen-2024-Iswanti.pdf
  content_type: application/pdf
  size: 4444554
  md5: 2b2cd519666c339ef5d08ce82310daef

## Thumbnail

fileset_id: abd3be10-780e-4da3-bb54-e16cc1426ac8
filename: Lysosomal enzymes and the oxygen-2024-Iswanti.pdf