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説明:

Volumetric muscle loss (VML) is a traumatic or surgical injury to the skeletal muscles that causes irrecoverable functional loss leading to chronic deficits and long-term disability. Although cell transplantation is a potent therapeutic approach, treating VML remains challenging because of the poor graft survival of cell suspensions injected into defects. Here, we report the development of tunable micropore-forming injectable hydrogels to deliver mesenchymal stem cells (MSCs) for VML treatment. The molecular modification of gelatin with hydrogen-bonding functional groups induces liquid-liquid phase separation when mixed with chemically crosslinkable gelatin to form injectable hydrogels with tunable microporous structures. MSCs encapsulated in porous hydrogels show higher cell adhesion, spreading, proliferation, and secretion of paracrine signals than those encapsulated in non-porous hydrogels. Porous hydrogels enhance cell infiltration and myoblast differentiation Additionally, porous hydrogels improve the graft survival of transplanted MSCs in VML mouse models and ameliorate therapeutic efficiency. This controlled microstructure-containing injectable hydrogel may serve as a cell-delivering scaffold to improve the efficacy of cell transplantation therapies in regenerative medicine.

権利情報:

• Creative Commons BY-NC-ND Attribution-NonCommercial-NoDerivs 4.0 International
  

キーワード: Regenerative medicine, Hydrogel, Liquid-liquid phase separation, Porous material, Mechanobiology

刊行年月日: 2025-06-25

出版者: Advanced Functional Materials

掲載誌:

• ADVANCED FUNCTIONAL MATERIALS (ISSN: 16163028)

研究助成金:

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.1002/adfm.202508278

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更新時刻: 2025-06-27 17:03:30 +0900

MDRでの公開時刻: 2025-06-27 16:19:09 +0900