# Incorporation of Triacylglycerol and Cholesteryl Ester Droplets in Phase-Separated Giant Unilamellar Vesicles

https://mdr.nims.go.jp/datasets/87e158fc-9cba-435f-a312-6a1b72e8b507

## Files

- [著者最終稿_Languir2025.pdf](https://mdr.nims.go.jp/filesets/c2c983af-bd0b-49ba-a3ac-cfc5d1b455c0/download) ([Detail](https://mdr.nims.go.jp/filesets/c2c983af-bd0b-49ba-a3ac-cfc5d1b455c0.md))
- [Supporting_information.pdf](https://mdr.nims.go.jp/filesets/c8026c7f-cf2c-4c16-93ed-e2ecb9fad4da/download) ([Detail](https://mdr.nims.go.jp/filesets/c8026c7f-cf2c-4c16-93ed-e2ecb9fad4da.md))

## Id

87e158fc-9cba-435f-a312-6a1b72e8b507

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-03-12T03:51:54.485657Z

## Updated at

2026-02-26T03:30:10.042958Z

## Published at

2026-02-26T00:41:06.212126Z

## Doi

https://doi.org/10.48505/nims.5365

## First published url

https://doi.org/10.1021/acs.langmuir.4c05063

## Date published

2025-03-11

## Recorded date published

2025-3-11

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Incorporation of Triacylglycerol and Cholesteryl Ester Droplets in Phase-Separated
    Giant Unilamellar Vesicles
  title_type: original
  lang: en

## Description

- description: In this study, we used fluorescence microscopy to investigate the incorporation
    of droplets composed of triolein, trilinolein, trimyristolein, trieicosenoin,
    and cholesteryl arachidonate in the bilayers of giant unilamellar vesicles (GUVs)
    consisting of a mixture of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
    (DPPC), and cholesterol. After incorporating the triacylglycerol droplets, the
    DOPC/DPPC/cholesterol (3:3:2) GUVs, which exhibited liquid-disordered (Ld) and
    liquid-ordered (Lo) phase separation, retained their phase-separated state. The
    triacylglycerol droplets predominantly partitioned in the Ld domains. To elucidate
    the basis of this preferential partitioning, we investigated the surface pressures
    of DOPC, DPPC, and cholesterol monolayers containing triolein at the air–water
    interface using a Langmuir trough. From these measurements, we determined the
    interfacial tension at the monolayer-covered triolein–water interface. The results
    showed that DOPC most effectively reduced the interfacial tension. Thus, the droplet
    sorting into the DOPC-enriched Ld domains likely arose from the difference in
    the abilities of the two phases to stabilize the droplet interface. In contrast,
    cholesteryl arachidonate had a profound effect on the bilayer phase behavior.
    Fluorescence images of the DOPC/DPPC/cholesterol (3:3:2) GUVs showed that the
    domain structures disappeared after droplet incorporation. Additionally, surface
    pressure measurements of DOPC/DPPC/cholesterol (3:3:2) monolayers containing cholesteryl
    arachidonate at the air–water interface suggested that cholesteryl arachidonate
    weakened the lipid–lipid interaction. The results indicate that the cholesteryl
    arachidonate molecules diffused across the bilayer to hinder bilayer phase separation.
  description_type: abstract
  lang: und

## Creator

- name: Chiho Kataoka-Hamai
  role: author
  orcid: https://orcid.org/0000-0002-4068-0405
- name: Jingwen Song
  role: author
  orcid: https://orcid.org/0000-0003-1910-9287
- name: Kohsaku Kawakami
  role: author
  orcid: https://orcid.org/0000-0002-3466-9365

## Contact agent



## Publisher

organization: American Chemical Society (ACS)

## Managing organization



## Keyword

- subject: Lipid droplets
  schema: not_defined
- subject: triacylglycerols
  schema: not_defined
- subject: giant unilamellar vesicles
  schema: not_defined
- subject: GUVs
  schema: not_defined

## Rights

- description: This document is the Accepted Manuscript version of a Published Work
    that appeared in final form in Langmuir, copyright © 2025 American Chemical Society
    after peer review and technical editing by the publisher. To access the final
    edited and published work see https://doi.org/10.1021/acs.langmuir.4c05063.
  identifier: http://rightsstatements.org/vocab/InC/1.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2025-02-26
end_date: 2026-02-26

## Journal

- title: Langmuir
  issn: '07437463'
  volume: '41'
  issue: '9'

## Conference



## Related item



## Funding

- identifier: 22K05179
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Custom property



## Fileset

- id: c2c983af-bd0b-49ba-a3ac-cfc5d1b455c0
  filename: 著者最終稿_Languir2025.pdf
  content_type: application/pdf
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  md5: 5dd269f00cf9ef1eccce18814f849c2b
- id: c8026c7f-cf2c-4c16-93ed-e2ecb9fad4da
  filename: Supporting_information.pdf
  content_type: application/pdf
  size: 234415
  md5: b6d8a251528e40b756d216105b8c1b5b

## Thumbnail

fileset_id: c2c983af-bd0b-49ba-a3ac-cfc5d1b455c0
filename: 著者最終稿_Languir2025.pdf