# Isolation and Characterization of Human Gut Bacteria Capable of Extracellular Electron Transport by Electrochemical Techniques

https://mdr.nims.go.jp/datasets/87d3abd3-88df-4627-9d16-0b3604bc6464

## File

- [fmicb-09-03267.pdf](https://mdr.nims.go.jp/filesets/3ac7ccee-9b4a-451b-9e40-47d9af06f378/download) ([Detail](https://mdr.nims.go.jp/filesets/3ac7ccee-9b4a-451b-9e40-47d9af06f378.md))

## Id

87d3abd3-88df-4627-9d16-0b3604bc6464

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-12-16T04:50:57.693945Z

## Updated at

2024-12-18T07:30:52.322189Z

## Published at

2024-12-18T07:30:52.381055Z

## Doi



## First published url

https://doi.org/10.3389/fmicb.2018.03267

## Date published

2019-01-15

## Recorded date published



## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Isolation and Characterization of Human Gut Bacteria Capable of Extracellular
    Electron Transport by Electrochemical Techniques
  title_type: original
  lang: en

## Description

- description: "Microorganisms are known to exhibit extracellular electron transfer
    (EET) in a wide variety of habitats. However, as for the human microbiome which
    significantly impacts our health, the role and importance of EET has not been
    widely investigated. In this study, we enriched and isolated the EET-capable bacteria
    from human gut microbes using an electrochemical enrichment method and examined
    whether the isolates couple EET with anaerobic respiration or fermentation. Upon
    the use of energy-rich or minimum media (with acetate or lactate) for electrochemical
    enrichment with the human gut sample at an electrode potential of (+0.4 V vs SHE),
    both culture conditions showed significant current production. However, EET-capable
    pure strains were enriched specifically with minimum media, and subsequent incubation
    using the \U0001D6FF-MnO2-agar plate with lactate or acetate led to the isolation
    of two EET-capable microbial strains, Gut-S1 and Gut-S2, having 99.9% of 16S rRNA
    gene sequence identity with Enterococcus avium (E. avium) and Klebsiella pneumoniae,
    (K. pneumoniae) respectively. While the enrichment involved anaerobic respiration
    with acetate and lactate, further electrochemistry with E. avium and K. pneumoniae
    revealed that the glucose fermentation was also coupled with EET. These results
    indicate that EET couples not only with anaerobic respiration as found in environmental
    bacteria, but also with fermentation in the human gut. "
  description_type: abstract
  lang: und

## Creator

- name: Divya Naradasu
  role: author
  organization: National Institute for Materials Science
- name: Waheed Miran
  role: author
- name: Mitsuo Sakamoto
  role: author
- name: Akihiro Okamoto
  role: author
  orcid: https://orcid.org/0000-0002-8102-4316
  organization: National Institute for Materials Science

## Contact agent



## Publisher

organization: Frontiers Media SA

## Managing organization



## Keyword

- subject: whole-cell electrochemistry
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Frontiers in Microbiology
  issn: 1664302X
  volume: '9'

## Conference



## Related item



## Funding

- identifier: JP18gm6010002
  funder_name: Japan Agency for Medical Research and Development
- identifier: JP18gm6010007
  funder_name: Japan Agency for Medical Research and Development
- identifier: 17H04969
  funder_name: Japan Society for the Promotion of Science
- identifier: N62909-17-1-2038
  funder_name: Office of Naval Research Global

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 3ac7ccee-9b4a-451b-9e40-47d9af06f378
  filename: fmicb-09-03267.pdf
  content_type: application/pdf
  size: 2597353
  md5: 623ba327255570e96dff232d7a2598c2

## Thumbnail

fileset_id: 3ac7ccee-9b4a-451b-9e40-47d9af06f378
filename: fmicb-09-03267.pdf