# Inhaled nitric oxide as a rescue therapy in rat crush syndrome: translating bench research to field application

https://mdr.nims.go.jp/datasets/795969be-882c-4ae6-a868-2c710e1a4b87

## File

- [2026.03.09.710439v1.full.pdf](https://mdr.nims.go.jp/filesets/0c2a9c7c-1bd8-42a8-9a12-ab07fac3d648/download) ([Detail](https://mdr.nims.go.jp/filesets/0c2a9c7c-1bd8-42a8-9a12-ab07fac3d648.md))

## Id

795969be-882c-4ae6-a868-2c710e1a4b87

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2026-04-14T08:04:55.686174Z

## Updated at

2026-04-22T00:36:06.526444Z

## Published at

2026-04-22T03:24:42.235053Z

## Doi



## First published url

https://doi.org/10.64898/2026.03.09.710439

## Date published

2026-03-12

## Recorded date published



## Resource type

journal_article

## Manuscript type

authors_original

## Collection



## Title

- title: 'Inhaled nitric oxide as a rescue therapy in rat crush syndrome: translating
    bench research to field application'
  title_type: original
  lang: en

## Description

- description: Crush syndrome (CS) is characterised by ischaemia/reperfusion-induced
    rhabdomyolysis, leading to systemic inflammation and high mortality. Building
    on our previous findings that intravenous nitric oxide (NO) donors improve survival
    in this condition, we investigated the therapeutic efficacy of inhaled NO delivered
    via a portable, controlled-release device in an experimental rat model of CS.
    Anaesthetised rats underwent bilateral hindlimb compression using rubber tourniquets
    for 5 h, followed by reperfusion. Among the various inhalation conditions tested,
    administration of NO (160 parts per million) for 2 h after reperfusion significantly
    increased survival rate from 20 to 90%. Improvements in haemodynamic parameters,
    biochemical markers, and histopathological findings correlated with enhanced survival
    outcomes. These results suggest that on-site NO inhalation therapy may serve as
    an effective first-line, emergency intervention for CS, particularly in disaster
    settings.
  description_type: abstract
  lang: und

## Creator

- name: Isamu Murata
  role: author
- name: Jun Kobayashi
  role: author
- name: Shinsuke Ishihara
  role: author
  orcid: https://orcid.org/0000-0001-6854-6032
  organization: National Institute for Materials Science
- name: Nobuo Iyi
  role: author
  orcid: https://orcid.org/0000-0001-5547-7031
  organization: National Institute for Materials Science

## Contact agent



## Publisher

organization: bioRxiv

## Managing organization



## Keyword

- subject: Crash syndrome
  schema: not_defined
- subject: Nitric oxide
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/
  date_licensed: 2026-03-12

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: bioRxiv
  start_page: 1
  end_page: 32

## Conference



## Related item



## Funding

- identifier: JP22K09148
  funder_name: SPS KAKENHI

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 0c2a9c7c-1bd8-42a8-9a12-ab07fac3d648
  filename: 2026.03.09.710439v1.full.pdf
  content_type: application/pdf
  size: 1903602
  md5: 1623542f85aa9fb85c5067c8fe752690

## Thumbnail

fileset_id: 0c2a9c7c-1bd8-42a8-9a12-ab07fac3d648
filename: 2026.03.09.710439v1.full.pdf