# Alkyl anchor–modified artificial viral capsid budding outside-to-inside and inside-to-outside giant vesicles

https://mdr.nims.go.jp/datasets/77a09f52-fe4b-4340-93d9-b3ea7057f314

## File

- [Alkyl anchor modified artificial viral capsid budding outside-to-inside and inside-to-outside giant vesicles.pdf](https://mdr.nims.go.jp/filesets/d0ed37eb-be7e-459e-9e2b-0a770ea905fa/download) ([Detail](https://mdr.nims.go.jp/filesets/d0ed37eb-be7e-459e-9e2b-0a770ea905fa.md))

## Id

77a09f52-fe4b-4340-93d9-b3ea7057f314

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-05-02T01:21:55.331636Z

## Updated at

2024-05-08T07:30:23.198110Z

## Published at

2024-05-08T07:30:23.303628Z

## Doi

https://doi.org/10.48505/nims.4499

## First published url

https://doi.org/10.1080/14686996.2024.2347191

## Date published

2024-12-31

## Recorded date published

2024-12-31

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Alkyl anchor–modified artificial viral capsid budding outside-to-inside and
    inside-to-outside giant vesicles
  title_type: original
  lang: en

## Description

- description: The budding of human immunodeficiency virus from an infected host cell
    is induced by the modification of structural proteins bearing long-chain fatty
    acids, followed by their anchoring to the cell membrane. Although many model budding
    systems using giant unilamellar vesicles (GUVs) induced by various stimuli have
    been developed, constructing an artificial viral budding system of GUVs using
    only synthesized molecules remains challenging. Herein, we report the construction
    of an artificial viral capsid budding system from a lipid bilayer of GUV. The
    C-terminus of the β-annulus peptide was modified using an octyl chain as an alkyl
    anchor via a disulfide bond. The self-assembly of the β-annulus peptide with an
    octyl chain formed an artificial viral capsid aggregate. The fluorescence imaging
    and transmission electron microscopy observations revealed that the addition of
    the tetramethylrhodamine (TMR)-labeled octyl chain – bearing β-annulus peptide
    to the outer aqueous phase of GUV induced the budding of the capsid-encapsulated
    daughter vesicle outside-to-inside the mother GUV. Conversely, the encapsulation
    of the TMR-labeled octyl chain – bearing β-annulus peptide in the inner aqueous
    phase of GUV induced the budding of the capsid-encapsulated daughter vesicle inside-to-outside
    the mother GUV. Contrarily, the addition of the TMR-labeled β-annulus peptide
    to GUV barely induced budding. It was demonstrated that the higher the membrane
    fluidity of GUV, the more likely budding would be induced by the addition of the
    alkyl anchor – modified artificial viral capsid. The simple virus-mimicking material
    developed in this study, which buds off through membrane anchoring, can provide
    physicochemical insights into the mechanisms of natural viral budding from cells.
  description_type: abstract
  lang: en

## Creator

- name: Kazunori Matsuura
  role: author
  organization: Tottori University
  department: Graduate School of Engineering
- name: Miu Hirahara
  role: author
- name: Kentarou Sakamoto
  role: author
  organization: Malaysia–Japan International Institute of Technology
- name: Hiroshi Inaba
  role: author

## Contact agent



## Publisher

organization: Taylor & Francis

## Managing organization



## Keyword

- subject: Artificial viral capsid
  schema: not_defined
- subject: self-assembly
  schema: not_defined
- subject: β-annulus peptide
  schema: not_defined
- subject: alkyl anchor
  schema: not_defined
- subject: budding
  schema: not_defined
- subject: giant vesicle
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Science and Technology of Advanced Materials
  issn: '14686996'
  volume: '25'
  article_number: '2347191'

## Conference



## Related item



## Funding



## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: d0ed37eb-be7e-459e-9e2b-0a770ea905fa
  filename: Alkyl anchor modified artificial viral capsid budding outside-to-inside
    and inside-to-outside giant vesicles.pdf
  content_type: application/pdf
  size: 7940869
  md5: bf590ad5eeabaf397fc1cc450bf43a3e

## Thumbnail

fileset_id: d0ed37eb-be7e-459e-9e2b-0a770ea905fa
filename: Alkyl anchor modified artificial viral capsid budding outside-to-inside
  and inside-to-outside giant vesicles.pdf