# Effects of Synthetic Toll-Like Receptor 9 Ligand Molecules on Pulpal Immunomodulatory Response and Repair after Injuries

https://mdr.nims.go.jp/datasets/6bcb73d3-df2b-4c97-b074-9bcc47e64e4c

## File

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## Id

6bcb73d3-df2b-4c97-b074-9bcc47e64e4c

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-11-15T07:53:09.348298Z

## Updated at

2024-11-19T07:31:27.708719Z

## Published at

2024-11-19T07:31:27.790429Z

## Doi



## First published url

https://doi.org/10.3390/biom14080931

## Date published

2024-08-01

## Recorded date published



## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Effects of Synthetic Toll-Like Receptor 9 Ligand Molecules on Pulpal Immunomodulatory
    Response and Repair after Injuries
  title_type: original
  lang: en

## Description

- description: Synthetic oligodeoxynucleotides (ODNs) containing unmethylated cytosine-phosphate-guanine
    (CpG) motifs (CpG-ODNs) are ligand molecules for Toll-like receptor 9 (TLR9),
    which is expressed by odontoblasts in vitro and dental pulp cells. This study
    determined the effects of CpG-ODNs on pulpal immunomodulatory response and repair
    following injury. Briefly, the upper right first molars of three-week-old mice
    were extracted, immersed in Type A (D35) or B (K3) CpG-ODN solutions (0.1 or 0.8
    mM) for 30 min, and then replanted. Pulpal healing and immunomodulatory activity
    were assessed by hematoxylin-eosin and AZAN staining, as well as immunohistochemistry.
    One week following the operation, inflammatory reactions occurred in all of the
    experimental groups; however, re-revascularization and newly formed hard tissue
    deposition were observed in the pulp chamber of all groups at week 2. A positive
    trend in the expression of immune cell markers was observed toward the CpG-ODN
    groups at 0.1 mM. Our data suggest that synthetic CpG-ODN solutions at low concentrations
    may evoke a long-lasting macrophage-TLR9-mediated pro-inflammatory, rather than
    anti-inflammatory, response in the dental pulp to modulate the repair process
    and hard tissue formation. Further studies are needed to determine the effects
    of current immunomodulatory agents in vitro and in vivo and develop treatment
    strategies for dental tissue regeneration.
  description_type: abstract
  lang: und

## Creator

- name: Angela Quispe-Salcedo
  role: author
- name: Tomohiko Yamazaki
  role: author
  orcid: https://orcid.org/0000-0003-2136-8042
  organization: National Institute for Materials Science
- name: Hayato Ohshima
  role: author

## Contact agent



## Publisher

organization: MDPI AG

## Managing organization



## Keyword

- subject: dental pulp
  schema: not_defined
- subject: CpG oligodeoxynucleotide (CpG ODN)
  schema: not_defined
- subject: Toll-like receptor 9 (TLR9)
  schema: not_defined
- subject: mice
  schema: not_defined
- subject: odontoblasts
  schema: not_defined
- subject: tooth replantation
  schema: not_defined
- subject: tooth injuries
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Biomolecules
  issn: 2218273X
  volume: '14'
  issue: '8'
  article_number: '931'

## Conference



## Related item



## Funding

- identifier: P20412
  funder_name: Japan Society for the Promotion of Science
  description: 日本学術振興会、JSPS
- identifier: 22K21011
  funder_name: JSPS KAKENHI
  description: 日本学術振興会、JSPS
- identifier: 23H03078
  funder_name: JSPS KAKENHI
  description: 日本学術振興会、JSPS

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: f3df3576-2dcf-4724-b341-fd958521c255
  filename: Effects of Synthetic Toll--2024-Quispe-Salcedo.pdf
  content_type: application/pdf
  size: 1840949
  md5: 6db38057c2f5acbf126351285017017c
- id: c649ce23-814a-4b5e-a985-f77d7274882b
  filename: Effects of Synthetic Toll--2024-Quispe-Salced1.pdf
  content_type: application/pdf
  size: 1088514
  md5: ee36316b38674c4d13dc825072af59bc

## Thumbnail

fileset_id: c649ce23-814a-4b5e-a985-f77d7274882b
filename: Effects of Synthetic Toll--2024-Quispe-Salced1.pdf