# Focal adhesion and actin orientation regulated by cellular geometry determine stem cell differentiation via mechanotransduction

https://mdr.nims.go.jp/datasets/6a071c76-ff77-4d4b-a11c-a5ba138a7fc5

## File

- [Focal Adhesion and Actin Orientation Regulated by Cellular Geometry Determine Stem Cell Differentiation via Mechanotransduction.docx](https://mdr.nims.go.jp/filesets/db4af03c-bfe7-4736-9a54-04f2a355bcf0/download) ([Detail](https://mdr.nims.go.jp/filesets/db4af03c-bfe7-4736-9a54-04f2a355bcf0.md))

## Id

6a071c76-ff77-4d4b-a11c-a5ba138a7fc5

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-10-09T02:05:32.958079Z

## Updated at

2024-10-10T00:57:56.096951Z

## Published at

2026-05-08T23:39:04.392859Z

## Doi

https://doi.org/10.48505/nims.4828

## First published url

https://doi.org/10.1016/j.actbio.2024.05.017

## Date published

2024-05-09

## Recorded date published

2024-7

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Focal adhesion and actin orientation regulated by cellular geometry determine
    stem cell differentiation via mechanotransduction
  title_type: original
  lang: en

## Description

- description: 'Tuning cell adhesion geometry can affect cytoskeleton organization
    and the distribution of cytoskeleton forces, which play critical roles in controlling
    cell functions. To elucidate the geometrical relationship with cytoskeleton force
    distribution, it is necessary to control cell morphology. In this study, a series
    of dextral vortex micropatterns were prepared to precisely control cell morphology
    for investigating the influence of the curvature degree of adhesion curves on
    intracellular force distribution and stem cell differentiation at a sub-cellular
    level. Peripherial actin filaments of micropatterned cells were assembled along
    the adhesion curves and showed different orientations, filament thicknesses and
    densities. Focal adhesion and cytoskeleton force distribution were dependent on
    the curvature degree. Intracellular force distribution was also regulated by adhesion
    curves. The cytoskeleton and force distribution affected the osteogenic differentiation
    of mesenchymal stem cells through a YAP/TAZ-mediated mechanotransduction process.
    Thus, regulation of cell adhesion curvature, especially at cytoskeletal filament
    level, is critical for cell function manipulation. STATEMENT OF SIGNIFICANCE:
    In this study, a series of dextral micro-vortexes were prepared and used for the
    culture of human mesenchymal stem cells (hMSCs) to precisely control adhesive
    curvatures (0°, 30°, 60°, and 90°). The single MSCs on the micropatterns had the
    same size and shape but showed distinct focal adhesion (FA) and cytoskeleton orientations.
    Cellular nanomechanics were observed to be correlated with the curvature degrees,
    subsequently influencing nuclear morphological features. As a consequence, the
    localization of the mechanotransduction sensor and activator-YAP/TAZ was affected,
    influencing osteogenic differentiation. The results revealed the pivotal role
    of adhesive curvatures in the manipulation of stem cell differentiation via the
    machanotransduction process, which has rarely been investigated.'
  description_type: abstract
  lang: und

## Creator

- name: Xinlong Wang
  role: author
  orcid: https://orcid.org/0000-0001-8978-2851
- name: Yingjun Yang
  role: author
  orcid: https://orcid.org/0000-0003-2746-1192
- name: Yongtao Wang
  role: author
- name: Chengyu Lu
  role: author
- name: Xiaohong Hu
  role: author
- name: Naoki Kawazoe
  role: author
  orcid: https://orcid.org/0000-0003-3916-0709
- name: Yingnan Yang
  role: author
- name: Guoping Chen
  role: author
  orcid: https://orcid.org/0000-0001-6753-3678

## Contact agent



## Publisher

organization: Elsevier BV

## Managing organization



## Keyword

- subject: Adhesion geometry
  schema: not_defined
- subject: Curvature degree
  schema: not_defined
- subject: Cytoskeleton force
  schema: not_defined
- subject: Micropattern
  schema: not_defined
- subject: Mesenchymal stem cells
  schema: not_defined
- subject: Osteogenic differentiation
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2024-05-09
end_date: 2026-05-10

## Journal

- title: Acta Biomaterialia
  issn: '17427061'
  volume: '182'
  start_page: 81
  end_page: 92

## Conference



## Related item



## Funding

- identifier: 22K19926
  funder_name: Japan Society for the Promotion of Science
- identifier: 21H03830
  funder_name: Japan Society for the Promotion of Science
- identifier: 24K03289
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: db4af03c-bfe7-4736-9a54-04f2a355bcf0
  filename: Focal Adhesion and Actin Orientation Regulated by Cellular Geometry Determine
    Stem Cell Differentiation via Mechanotransduction.docx
  content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
  size: 6987957
  md5: 7f6a608d59b661315cdf1b4a861e4bd7

## Thumbnail

fileset_id: db4af03c-bfe7-4736-9a54-04f2a355bcf0
filename: Focal Adhesion and Actin Orientation Regulated by Cellular Geometry Determine
  Stem Cell Differentiation via Mechanotransduction.docx