# Effect of Hydrogel Stiffness on Chemoresistance of Breast Cancer Cells in 3D Culture

https://mdr.nims.go.jp/datasets/62490f30-afe4-4f99-965a-ae1433c7efc9

## File

- [Effect of Hydrogel Stiffness on Chemoresistance of Breast Cancer Cells in 3D Culture.pdf](https://mdr.nims.go.jp/filesets/aaddac17-e054-4529-8ec6-59205e687cc2/download) ([Detail](https://mdr.nims.go.jp/filesets/aaddac17-e054-4529-8ec6-59205e687cc2.md))

## Id

62490f30-afe4-4f99-965a-ae1433c7efc9

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-10-09T02:28:06.123572Z

## Updated at

2024-10-10T07:30:43.375291Z

## Published at

2024-10-10T07:30:43.457001Z

## Doi



## First published url

https://doi.org/10.3390/gels10030202

## Date published

2024-03-17

## Recorded date published



## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Effect of Hydrogel Stiffness on Chemoresistance of Breast Cancer Cells in
    3D Culture
  title_type: original
  lang: en

## Description

- description: Chemotherapy is one of the most common strategies for cancer treatment,
    whereas drug resistance reduces the efficiency of chemotherapy and leads to treatment
    failure. The mechanism of emerging chemoresistance is complex and the effect of
    extracellular matrix (ECM) surrounding cells may contribute to drug resistance.
    Although it is well known that ECM plays an important role in orchestrating cell
    functions, it remains exclusive how ECM stiffness affects drug resistance. In
    this study, we prepared agarose hydrogels of different stiffnesses to investigate
    the effect of hydrogel stiffness on the chemoresistance of breast cancer cells
    to doxorubicin (DOX). Agarose hydrogels with a stiffness range of 1.5 kPa to 112.3
    kPa were prepared and used to encapsulate breast cancer cells for a three-dimensional
    culture with different concentrations of DOX. The viability of the cells cultured
    in the hydrogels was dependent on both DOX concentration and hydrogel stiffness.
    Cell viability decreased with DOX concentration when the cells were cultured in
    the same stiffness hydrogels. When DOX concentration was the same, breast cancer
    cells showed higher viability in high-stiffness hydrogels than they did in low-stiffness
    hydrogels. Furthermore, the expression of P-glycoprotein mRNA in high-stiffness
    hydrogels was higher than that in low-stiffness hydrogels. The results suggested
    that hydrogel stiffness could affect the resistance of breast cancer cells to
    DOX by regulating the expression of chemoresistance-related genes.
  description_type: abstract
  lang: und

## Creator

- name: Tianjiao Zeng
  role: author
  orcid: https://orcid.org/0000-0002-1286-0337
- name: Huajian Chen
  role: author
- name: Toru Yoshitomi
  role: author
  orcid: https://orcid.org/0000-0003-3847-1812
- name: Naoki Kawazoe
  role: author
  orcid: https://orcid.org/0000-0003-3916-0709
- name: Yingnan Yang
  role: author
- name: Guoping Chen
  role: author
  orcid: https://orcid.org/0000-0001-6753-3678

## Contact agent



## Publisher

organization: MDPI AG

## Managing organization



## Keyword

- subject: agarose hydrogel
  schema: not_defined
- subject: stiffness
  schema: not_defined
- subject: chemoresistance
  schema: not_defined
- subject: breast cancer cells
  schema: not_defined
- subject: 3D culture
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin



## Embargo



## Journal

- title: Gels
  issn: '23102861'
  volume: '10'
  issue: '3'
  start_page: 202

## Conference



## Related item



## Funding

- identifier: 19H04475
  funder_name: JSPS KAKENHI
- identifier: 21H03830
  funder_name: JSPS KAKENHI
- identifier: 22K19926
  funder_name: JSPS KAKENHI

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Fileset

- id: aaddac17-e054-4529-8ec6-59205e687cc2
  filename: Effect of Hydrogel Stiffness on Chemoresistance of Breast Cancer Cells
    in 3D Culture.pdf
  content_type: application/pdf
  size: 4554176
  md5: 510495299dbf4522963a2aa6f3f3ffb9

## Thumbnail

fileset_id: aaddac17-e054-4529-8ec6-59205e687cc2
filename: Effect of Hydrogel Stiffness on Chemoresistance of Breast Cancer Cells in
  3D Culture.pdf