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Chemotherapy is one of the most common strategies for cancer treatment, whereas drug resistance reduces the efficiency of chemotherapy and leads to treatment failure. The mechanism of emerging chemoresistance is complex and the effect of extracellular matrix (ECM) surrounding cells may contribute to drug resistance. Although it is well known that ECM plays an important role in orchestrating cell functions, it remains exclusive how ECM stiffness affects drug resistance. In this study, we prepared agarose hydrogels of different stiffnesses to investigate the effect of hydrogel stiffness on the chemoresistance of breast cancer cells to doxorubicin (DOX). Agarose hydrogels with a stiffness range of 1.5 kPa to 112.3 kPa were prepared and used to encapsulate breast cancer cells for a three-dimensional culture with different concentrations of DOX. The viability of the cells cultured in the hydrogels was dependent on both DOX concentration and hydrogel stiffness. Cell viability decreased with DOX concentration when the cells were cultured in the same stiffness hydrogels. When DOX concentration was the same, breast cancer cells showed higher viability in high-stiffness hydrogels than they did in low-stiffness hydrogels. Furthermore, the expression of P-glycoprotein mRNA in high-stiffness hydrogels was higher than that in low-stiffness hydrogels. The results suggested that hydrogel stiffness could affect the resistance of breast cancer cells to DOX by regulating the expression of chemoresistance-related genes.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: agarose hydrogel, stiffness, chemoresistance, breast cancer cells, 3D culture

刊行年月日: 2024-03-17

出版者: MDPI AG

掲載誌:

• Gels (ISSN: 23102861) vol. 10 issue. 3 p. 202-

研究助成金:

• JSPS KAKENHI 19H04475
• JSPS KAKENHI 21H03830
• JSPS KAKENHI 22K19926

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.3390/gels10030202

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更新時刻: 2024-10-10 16:30:43 +0900

MDRでの公開時刻: 2024-10-10 16:30:43 +0900