# Turning on Protein Function Inhibited by DNA Aptamers Employing a Covalent DNA-Binding Protein

https://mdr.nims.go.jp/datasets/609b2d57-2041-4f51-8d1e-7e0c1c3c3727

## File

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## Id

609b2d57-2041-4f51-8d1e-7e0c1c3c3727

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2026-06-24T09:46:02.752756Z

## Updated at

2026-06-25T00:39:07.515956Z

## Published at

2026-06-25T03:26:54.749565Z

## Doi



## First published url

https://doi.org/10.1021/acsnanoscienceau.5c00143

## Date published

2026-04-15

## Recorded date published

2026-4-15

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Turning on Protein Function Inhibited by DNA Aptamers Employing a Covalent
    DNA-Binding Protein
  title_type: original
  lang: en

## Description

- description: Certain DNA aptamers can serve as effective inhibitors, although their
    inhibitory action is typically unidirectional. Simple removal of these aptamers
    from their target complexes could improve their application in areas such as oligonucleotide
    therapeutics. In the current study, we used uracilDNA glycosylase from Mycobacterium
    smegmatis (UdgX), which binds　covalently to DNA after uracil removal. A suitable
    site for incorporating uracil-DNA into a G-quadruplex-structured DNA aptamer was
    identified, and subsequent restoration of thrombin activity previously inhibited
    by the DNA aptamers was examined. The addition of UdgX restored thrombin activity
    to nearly 100% within 1 min, demonstrating greater speed and efficacy than complementary
    strand addition targeting the thrombin aptamer. Furthermore, UdgX restored the
    binding capacity of the anti-VEGF antibody bevacizumab that had been inhibited
    by a hairpin-structured aptamer. These findings highlight the versatile potential
    of UdgX to turn on protein functions inhibited by DNA aptamers.
  description_type: abstract
  lang: und

## Creator

- name: Takeru Kanazu
  role: author
- name: Erika Komiya
  role: author
- name: Daimei Miura
  role: author
  orcid: https://orcid.org/0009-0007-0005-9976
- name: Kaori Tsukakoshi
  role: author
  orcid: https://orcid.org/0000-0003-4779-1113
- name: Kazunori Ikebukuro
  role: author
  orcid: https://orcid.org/0000-0003-2838-0562
- name: Tomohiko Yamazaki
  role: author
  orcid: https://orcid.org/0000-0003-2136-8042
- name: Ryutaro Asano
  role: author
  orcid: https://orcid.org/0000-0001-6795-8377

## Contact agent



## Publisher

organization: American Chemical Society (ACS)
ror: https://ror.org/

## Managing organization



## Keyword

- subject: Anticoagulant
  schema: not_defined
- subject: aptamer
  schema: not_defined
- subject: aptamer remover
  schema: not_defined
- subject: UdgX
  schema: not_defined
- subject: uracil-DNA glycosylase
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: ACS Nanoscience Au
  issn: '26942496'
  volume: '6'
  issue: '2'
  start_page: 201
  end_page: 207

## Conference



## Related item



## Funding

- identifier: 21K18321
  funder_name: Japan Society for the Promotion of Science
- funder_name: National Institute for Materials Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 72c7cedd-2860-4214-ac17-2abc0cd3a0b8
  filename: Turning on Protein Function Inhibi-2026-Kanazu.pdf
  content_type: application/pdf
  size: 4776776
  md5: 134c4f9f3f6f799224c822be82660ad3
- id: fac9f726-40d7-4547-ad4f-37ac6fa93eb8
  filename: Turning on Protein Function Inhibi-2026-Kanaz1.pdf
  content_type: application/pdf
  size: 1257499
  md5: c70b4c358c9282cd4c867d24456de2b1

## Thumbnail

fileset_id: 72c7cedd-2860-4214-ac17-2abc0cd3a0b8
filename: Turning on Protein Function Inhibi-2026-Kanazu.pdf