# Ionic Liquid Interface as a Cell Scaffold https://mdr.nims.go.jp/datasets/5f7f34c7-d88c-4392-aae0-57f2983b1fd0 ## File - [Advanced Materials - 2024 - Ueki - Ionic Liquid Interface as a Cell Scaffold.pdf](https://mdr.nims.go.jp/filesets/92d3eb46-dbd6-4b64-accc-03cd4086af72/download) ([Detail](https://mdr.nims.go.jp/filesets/92d3eb46-dbd6-4b64-accc-03cd4086af72.md)) ## Id 5f7f34c7-d88c-4392-aae0-57f2983b1fd0 ## Local identifier ## Visibility open_to_public ## State published ## Created at 2024-02-29T00:37:46.992263Z ## Updated at 2024-03-04T23:30:08.474257Z ## Published at 2024-03-04T23:30:08.872349Z ## Doi ## First published url https://doi.org/10.1002/adma.202310105 ## Date published 2024-02-26 ## Recorded date published 2024-6 ## Resource type journal_article ## Manuscript type vor ## Collection ## Title - title: Ionic Liquid Interface as a Cell Scaffold title_type: original lang: en ## Description - description: In sharp contrast to conventional solid/hydrogel platforms, water-immiscible liquids, such as perfluorocarbons and silicones, allow the adhesion of mammalian cells via protein nanolayers (PNLs) formed at the interface. However, fluorocarbons and silicones, which are typically used for liquid cell culture, possess only narrow ranges of physicochemical parameters and have not allowed for a wide variety of cell culturing environments. In this paper, it is proposed that water-immiscible ionic liquids (ILs) are a new family of liquid substrates with tunable physicochemical properties and high solvation capabilities. Tetraalkylphosphonium-based ILs are identified as non-cytotoxic ILs, whereon human mesenchymal stem cells are successfully cultured. By reducing the cation charge distribution, or ionicity, via alkyl chain elongation, the interface allows cell spreading with matured focal contacts. High-speed atomic force microscopy observations of the PNL formation process suggest that the cation charge distribution significantly altered the protein adsorption dynamics, which are associated with the degree of protein denaturation and the PNL mechanics. Moreover, by exploiting dissolution capability of ILs, anion-gel cell scaffold is fabricated. This enables to further identify the significant contribution of bulk subphase mechanics to cellular mechanosensing in liquid-based culture scaffolds. description_type: abstract lang: und ## Creator - name: Takeshi Ueki role: author orcid: https://orcid.org/0000-0001-9317-6280 organization: National Institute for Materials Science (NIMS) department: Research Center for Macromolecules and Biomaterials - name: Koichiro Uto role: author orcid: https://orcid.org/0000-0001-7091-0585 organization: National Institute for Materials Science (NIMS) department: Research Center for Macromolecules and Biomaterials - name: Shota Yamamoto role: author orcid: https://orcid.org/0000-0002-7422-0968 organization: National Institute for Materials Science (NIMS) department: Research Center for Macromolecules and Biomaterials - name: Ryota Tamate role: author orcid: https://orcid.org/0000-0002-1704-1058 organization: National Institute for Materials Science (NIMS) department: Research Center for Macromolecules and Biomaterials - name: Yuji Kamiyama role: author orcid: https://orcid.org/0000-0001-9483-2112 - name: Xiaofang Jia role: author orcid: https://orcid.org/0000-0003-1875-4655 - name: Hidenori Noguchi role: author orcid: https://orcid.org/0000-0001-9643-1689 organization: National Institute for Materials Science (NIMS) department: Research Center for Energy and Environmental Materials (GREEN) - name: Kosuke Minami role: author orcid: https://orcid.org/0000-0003-4145-1118 organization: National Institute for Materials Science (NIMS) department: Research Center for Macromolecules and Biomaterials - name: Katsuhiko Ariga role: author orcid: https://orcid.org/0000-0002-2445-2955 organization: National Institute for Materials Science (NIMS) department: Research Center for Materials Nanoarchitectonics (MANA) - name: Hongxin Wang role: author orcid: https://orcid.org/0000-0002-8984-0764 - name: Jun Nakanishi role: author orcid: https://orcid.org/0000-0003-4457-6581 organization: National Institute for Materials Science (NIMS) department: Research Center for Macromolecules and Biomaterials ## Contact agent ## Publisher organization: Wiley ## Managing organization ## Keyword - subject: ionic liquid schema: not_defined - subject: cell culture schema: not_defined - subject: mechanobiology schema: not_defined - subject: gels schema: not_defined - subject: liquid interface schema: not_defined - subject: stem cell schema: not_defined - subject: phosphonium schema: not_defined ## Rights - identifier: http://rightsstatements.org/vocab/InC/1.0/ ## Other identifier(s) ## Data origin - data_origin_type: other ## Embargo ## Journal - title: Advanced Materials issn: '09359648' article_number: '2310105' ## Conference ## Related item ## Funding - identifier: 20H02804 funder_name: JSPS description: to T.U. - identifier: 20K21229 funder_name: JSPS description: to T.U. - identifier: 23H02030 funder_name: JSPS description: to T.U. - identifier: 22K14705 funder_name: JSPS description: to S.Y. - identifier: 22H00596 funder_name: JSPS description: to J.N. - identifier: 23K17418 funder_name: JSPS description: to J.N. ## Instrument ## Instrument operator ## Instrument managing organization ## Measurement method ## Specimen ## Chemical composition ## Structure for specimen ## Structural feature for specimen ## Specific property for specimen ## Process for specimen treatment ## Computational method ## Energy level/transition state ## Software ## Custom property ## Fileset - id: 92d3eb46-dbd6-4b64-accc-03cd4086af72 filename: Advanced Materials - 2024 - Ueki - Ionic Liquid Interface as a Cell Scaffold.pdf content_type: application/pdf size: 6593671 md5: 0bacaa8a7bcfcea83d50e102540fc417 ## Thumbnail fileset_id: 92d3eb46-dbd6-4b64-accc-03cd4086af72 filename: Advanced Materials - 2024 - Ueki - Ionic Liquid Interface as a Cell Scaffold.pdf