# Triacylglycerol-droplet-induced bilayer spontaneous curvature in giant unilamellar vesicles

https://mdr.nims.go.jp/datasets/5d6b896c-bf82-4047-a273-56ed6c4267d6

## File

- [BiophysJ2024.pdf](https://mdr.nims.go.jp/filesets/ecce5c56-f4da-4744-96d3-278e506d4dcf/download) ([Detail](https://mdr.nims.go.jp/filesets/ecce5c56-f4da-4744-96d3-278e506d4dcf.md))
- [Supporting_information.pdf](https://mdr.nims.go.jp/filesets/e6346989-ea61-4f60-a070-6b63dee4864d/download) ([Detail](https://mdr.nims.go.jp/filesets/e6346989-ea61-4f60-a070-6b63dee4864d.md))

## Id

5d6b896c-bf82-4047-a273-56ed6c4267d6

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-07-02T23:43:56.719993Z

## Updated at

2025-11-18T07:30:03.944125Z

## Published at

2024-07-04T07:30:26.086495Z

## Doi



## First published url

https://doi.org/10.1016/j.bpj.2024.05.030

## Date published

2024-05-31

## Recorded date published

2024-7

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Triacylglycerol-droplet-induced bilayer spontaneous curvature in giant unilamellar
    vesicles
  title_type: original
  lang: en

## Description

- description: This study investigated the incorporation of triacylglycerol droplets
    in the bilayers of giant unilamellar vesicles (GUVs) using four triacylglycerols
    and four phosphatidylcholines by confocal laser scanning microscopy. The triacylglycerol
    droplets were incorporated between the monolayer leaflets of the GUVs. Among the
    spherical droplets protruding on only one side of the bilayers, the droplets bound
    to the outer leaflets outnumbered those bound to the inner leaflets. The more
    frequent droplet binding to the outer leaflet caused transbilayer asymmetry in
    the droplet surface density. A vesicle consisting of a single-bilayer spherical
    segment and a double-bilayer spherical segment was also observed. The yield of
    these vesicles was comparable to or higher than that of the droplet-incorporating
    GUVs for many of the phosphatidylcholine–triacylglycerol combinations. In a vesicle
    consisting of single-bilayer and double-bilayer segments, most of the triacylglycerol
    droplets were localized on the outermost membrane surface along the segment boundary
    and in the double-bilayer segment. To rationalize the formation of these vesicle
    structures, we propose that the transbilayer asymmetry in the droplet surface
    density induces spontaneous curvature of the bilayer, with the bilayer spontaneously
    bending away from the droplets. Energy calculations performed assuming the existence
    of spontaneous curvature of the bilayer corroborated the experimentally determined
    membrane shapes for the vesicles consisting of unilamellar and bilamellar regions.
  description_type: abstract
  lang: und

## Creator

- name: Chiho Kataoka-Hamai
  role: author
  orcid: https://orcid.org/0000-0002-4068-0405

## Contact agent



## Publisher

organization: Elsevier BV

## Managing organization



## Keyword

- subject: Lipid droplets
  schema: not_defined
- subject: Oil-water interface
  schema: not_defined
- subject: Giant unilamellar vesicles
  schema: not_defined
- subject: Phospholipids
  schema: not_defined
- subject: Triacylglycerols
  schema: not_defined
- subject: Spontaneous curvature
  schema: not_defined
- subject: Fluorescence microscopy
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Biophysical Journal
  issn: '00063495'
  volume: '123'
  issue: '13'

## Conference



## Related item



## Funding

- identifier: 22K05179
  funder_name: Japan Society for the Promotion of Science
  description: "油水界面のリン脂質単分子膜：構造の定量分析と生体膜の\r\n物理学"

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: ecce5c56-f4da-4744-96d3-278e506d4dcf
  filename: BiophysJ2024.pdf
  content_type: application/pdf
  size: 5816812
  md5: 3e165ab71c434655a5b12aa00195677e
- id: e6346989-ea61-4f60-a070-6b63dee4864d
  filename: Supporting_information.pdf
  content_type: application/pdf
  size: 426267
  md5: a182c87598c20707d9d8293db6fe9af2

## Thumbnail

fileset_id: ecce5c56-f4da-4744-96d3-278e506d4dcf
filename: BiophysJ2024.pdf