Download all files (5.49MB)

コレクション

引用

説明:

This study investigated the incorporation of triacylglycerol droplets in the bilayers of giant unilamellar vesicles (GUVs) using four triacylglycerols and four phosphatidylcholines by confocal laser scanning microscopy. The triacylglycerol droplets were incorporated between the monolayer leaflets of the GUVs. Among the spherical droplets protruding on only one side of the bilayers, the droplets bound to the outer leaflets outnumbered those bound to the inner leaflets. The more frequent droplet binding to the outer leaflet caused transbilayer asymmetry in the droplet surface density. A vesicle consisting of a single-bilayer spherical segment and a double-bilayer spherical segment was also observed. The yield of these vesicles was comparable to or higher than that of the droplet-incorporating GUVs for many of the phosphatidylcholine–triacylglycerol combinations. In a vesicle consisting of single-bilayer and double-bilayer segments, most of the triacylglycerol droplets were localized on the outermost membrane surface along the segment boundary and in the double-bilayer segment. To rationalize the formation of these vesicle structures, we propose that the transbilayer asymmetry in the droplet surface density induces spontaneous curvature of the bilayer, with the bilayer spontaneously bending away from the droplets. Energy calculations performed assuming the existence of spontaneous curvature of the bilayer corroborated the experimentally determined membrane shapes for the vesicles consisting of unilamellar and bilamellar regions.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: Lipid droplets, Oil-water interface, Giant unilamellar vesicles, Phospholipids, Triacylglycerols, Spontaneous curvature, Fluorescence microscopy

刊行年月日: 2024-05-31

出版者: Elsevier BV

掲載誌:

• Biophysical Journal (ISSN: 00063495) vol. 123 issue. 13

研究助成金:

• Japan Society for the Promotion of Science 22K05179 (油水界面のリン脂質単分子膜：構造の定量分析と生体膜の 物理学)

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.1016/j.bpj.2024.05.030

関連資料:

その他の識別子:

連絡先:

更新時刻: 2025-11-18 16:30:03 +0900

MDRでの公開時刻: 2024-07-04 16:30:26 +0900