# Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite for Anti-Infection Bone Void Fillers

https://mdr.nims.go.jp/datasets/562d5035-a959-4f12-8262-ac566d059602

## File

- [ijms-21-00551.pdf](https://mdr.nims.go.jp/filesets/d28ee057-1917-4ac8-af4a-4fe38b607896/download) ([Detail](https://mdr.nims.go.jp/filesets/d28ee057-1917-4ac8-af4a-4fe38b607896.md))

## Id

562d5035-a959-4f12-8262-ac566d059602

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-03-06T00:45:59.577377Z

## Updated at

2024-03-19T07:55:56.857500Z

## Published at

2024-03-19T07:55:56.920128Z

## Doi



## First published url

https://doi.org/10.3390/ijms21020551

## Date published

2020-01-15

## Recorded date published



## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Fabrication of Gentamicin-Loaded Hydroxyapatite/Collagen Bone-Like Nanocomposite
    for Anti-Infection Bone Void Fillers
  title_type: original
  lang: en

## Description

- description: Gentamicin-loaded hydroxyapatite/collagen bone-like nanocomposite (GNT
    HAp/Col) was fabricated for anti-infection bone void fillers and was evaluated
    its absorption desorption properties, antibacterial efficacy and cytotoxicity.
    Ten milligrams of the hydroxyapatite/collagen bone-like nanocomposite (HAp/Col)
    powder in 1 ml of the gentamicin sulfate (GNT) solution diluted with phosphate
    buffered saline (PBS) at 50 250 μg/ml were mixed at room temperature. After 6
    h mixing, GNT adsorption in all conditions reached plateau by Langmuir’s isotherm
    and maximum GNT adsorption amount was 34.1±7.2 μg in 250 μg/ml GNT solution. One-hundred
    milligrams of the fully GNT-loaded HAp/Col powder, soaked in 10 ml of PBS at 37
    °C, released all of GNT in 3 days. A shaking culture method for a GNT extraction
    from the GNT-HAp/Col and an inhibition zone assay for the GNT-HAp/Col compact
    showed antibacterial efficacy to Escherichia coli at least for 1 to 2 days. Further,
    no cytotoxicities were observed on MG 63 cells. Thus, the GNT-HAp/Col is a good
    candidate of bioresobable anti-infection bone void fillers by prevention initial
    infections which is primary cause of implant associate infection even for rapid
    bioresorbable materials.
  description_type: abstract
  lang: und

## Creator

- name: Sho Oshima
  role: author
  organization: National Institute for Materials Science
- name: Taira Sato
  role: author
- name: Michiyo Honda
  role: author
- name: Yasushi Suetsugu
  role: author
  orcid: https://orcid.org/0000-0002-8161-1908
  organization: National Institute for Materials Science
- name: Kazuhide Ozeki
  role: author
- name: Masanori Kikuchi
  role: author
  orcid: https://orcid.org/0000-0002-9451-8147
  organization: National Institute for Materials Science

## Contact agent



## Publisher

organization: MDPI AG

## Managing organization



## Keyword

- subject: antibiotic-impregnated biomaterial
  schema: not_defined
- subject: hydroxyapatite/collagen bone-like nanocomposite
  schema: not_defined
- subject: gentamicin sulfate
  schema: not_defined
- subject: bone void filler
  schema: not_defined
- subject: antibacterial activity
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: International Journal of Molecular Sciences
  issn: '14220067'
  volume: '21'
  issue: '5'
  article_number: '551'

## Conference



## Related item



## Funding



## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: d28ee057-1917-4ac8-af4a-4fe38b607896
  filename: ijms-21-00551.pdf
  content_type: application/pdf
  size: 6536302
  md5: 5ba278eac1fd00fee27140b180040f25

## Thumbnail

fileset_id: d28ee057-1917-4ac8-af4a-4fe38b607896
filename: ijms-21-00551.pdf