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Gentamicin-loaded hydroxyapatite/collagen bone-like nanocomposite (GNT HAp/Col) was fabricated for anti-infection bone void fillers and was evaluated its absorption desorption properties, antibacterial efficacy and cytotoxicity. Ten milligrams of the hydroxyapatite/collagen bone-like nanocomposite (HAp/Col) powder in 1 ml of the gentamicin sulfate (GNT) solution diluted with phosphate buffered saline (PBS) at 50 250 μg/ml were mixed at room temperature. After 6 h mixing, GNT adsorption in all conditions reached plateau by Langmuir’s isotherm and maximum GNT adsorption amount was 34.1±7.2 μg in 250 μg/ml GNT solution. One-hundred milligrams of the fully GNT-loaded HAp/Col powder, soaked in 10 ml of PBS at 37 °C, released all of GNT in 3 days. A shaking culture method for a GNT extraction from the GNT-HAp/Col and an inhibition zone assay for the GNT-HAp/Col compact showed antibacterial efficacy to Escherichia coli at least for 1 to 2 days. Further, no cytotoxicities were observed on MG 63 cells. Thus, the GNT-HAp/Col is a good candidate of bioresobable anti-infection bone void fillers by prevention initial infections which is primary cause of implant associate infection even for rapid bioresorbable materials.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: antibiotic-impregnated biomaterial, hydroxyapatite/collagen bone-like nanocomposite, gentamicin sulfate, bone void filler, antibacterial activity

刊行年月日: 2020-01-15

出版者: MDPI AG

掲載誌:

• International Journal of Molecular Sciences (ISSN: 14220067) vol. 21 issue. 5 551

研究助成金:

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.3390/ijms21020551

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更新時刻: 2024-03-19 16:55:56 +0900

MDRでの公開時刻: 2024-03-19 16:55:56 +0900