# Durable and biofunctional polydimethylsiloxane surfaces engineered with photocrosslinkable terpolymers for aligned and functional myotube formation

https://mdr.nims.go.jp/datasets/516d57f9-c65e-4f61-818f-f5da43b8716d

## File

- [Takagi .pdf](https://mdr.nims.go.jp/filesets/49ffbfb2-bf65-49e3-bf90-ffb35b10ffc5/download) ([Detail](https://mdr.nims.go.jp/filesets/49ffbfb2-bf65-49e3-bf90-ffb35b10ffc5.md))

## Id

516d57f9-c65e-4f61-818f-f5da43b8716d

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2026-01-14T05:08:26.407157Z

## Updated at

2026-01-14T06:07:38.593386Z

## Published at

2026-01-14T07:22:13.861215Z

## Doi



## First published url

https://doi.org/10.1016/j.bbiosy.2025.100126

## Date published

2025-12-29

## Recorded date published

2026-3

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Durable and biofunctional polydimethylsiloxane surfaces engineered with photocrosslinkable
    terpolymers for aligned and functional myotube formation
  title_type: original
  lang: en

## Description

- description: Polydimethylsiloxane (PDMS) is widely used in biomicrodevices owing
    to its excellent processability, flexibility, and optical properties. However,
    the poor cell adhesiveness of PDMS limits its application as a stable substrate
    for long-term cell cultures. To address these challenges, we synthesized a photocrosslinkable
    terpolymer composed of N-(2-hydroxypropyl)acrylamide, N-benzophenone acrylamide,
    and N-succinimidyl acrylate (NSA), and covalently grafted it onto PDMS surfaces
    using UV irradiation. The ternary polymer coatings exhibited long-term stability
    in aqueous media and suppressed thenonspecific adsorption of proteins and cell
    adhesion. Furthermore, the immobilization of collagen on the side groups of NSA
    provides selective cell-adhesive functionality. In particular, PDMS surfaces modified
    with a ternary polymer containing 10 mol% NSA supported the robust and sustained
    adhesion of C2C12 myoblasts. When combined with stripe-patterned microstructures,
    these surfaces promoted unidirectional alignment, efficient myotube formation,
    and strong expression of dystrophin, with the 25 μm-pitch pattern demonstrating
    the most pronounced effects. Notably, spontaneous contraction of the formed myotubes
    confirmed advanced functional differentiation. These results demonstrate that
    the proposed facile and durable surface modification strategy for PDMS imparts
    both anti-biofouling properties and selective biofunctionality. The PDMS modification
    strategy provides a versatile platform for engineering functional muscle fibers
    and expanding the potential of PDMS-based bio-microdevices and tissue-engineered
    constructs.
  description_type: abstract
  lang: und

## Creator

- name: Ryoma Takagi
  role: author
- name: Tadashi Nakaji-Hirabayashi
  role: author
  orcid: https://orcid.org/0000-0002-7562-3419
- name: Moe Kato
  role: author
- name: Miwako Shobo
  role: author
- name: Chiaki Yoshikawa
  role: author
  orcid: https://orcid.org/0000-0002-6589-387X

## Contact agent



## Publisher

organization: Elsevier BV

## Managing organization



## Keyword

- subject: Polydimethylsiloxane surface modification
  schema: not_defined
- subject: Photocrosslinkable polymer coating
  schema: not_defined
- subject: Bioinert–bioactive interface
  schema: not_defined
- subject: Collagen-functionalized surface
  schema: not_defined
- subject: Myoblast differentiation and alignment
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Biomaterials and Biosystems
  issn: '26665344'
  volume: '21'
  article_number: '100126'

## Conference



## Related item



## Funding

- funder_name: Government of Japan Ministry of Education Culture Sports Science and
    Technology

## Instrument



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## Measurement method



## Specimen



## Chemical composition



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## Fileset

- id: 49ffbfb2-bf65-49e3-bf90-ffb35b10ffc5
  filename: Takagi .pdf
  content_type: application/pdf
  size: 5661627
  md5: 3eede8dee9a03d891be1f3b2d1c1198a

## Thumbnail

fileset_id: 49ffbfb2-bf65-49e3-bf90-ffb35b10ffc5
filename: Takagi .pdf