# Development of Phase-Separating Microfiber Network Hydrogels to Promote <i>In Vitro</i> Vascularization

https://mdr.nims.go.jp/datasets/4e4d2631-9e88-4518-b5c7-356d1031bcad

## File

- [Manuscript-F.pdf](https://mdr.nims.go.jp/filesets/a0a6d53a-7812-4e25-add2-095e21f18003/download) ([Detail](https://mdr.nims.go.jp/filesets/a0a6d53a-7812-4e25-add2-095e21f18003.md))

## Id

4e4d2631-9e88-4518-b5c7-356d1031bcad

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-10-02T01:25:44.280011Z

## Updated at

2024-10-02T03:30:25.581965Z

## Published at

2024-10-02T03:30:25.671804Z

## Doi

https://doi.org/10.48505/nims.4789

## First published url

https://doi.org/10.1021/acs.biomac.4c00836

## Date published

2024-09-09

## Recorded date published

2024-9-9

## Resource type

journal_article

## Manuscript type

authors_original

## Collection



## Title

- title: Development of Phase-Separating Microfiber Network Hydrogels to Promote <i>In
    Vitro</i> Vascularization
  title_type: original
  lang: en

## Description

- description: Engineered vascularized tissues in vitro exhibit the potential for
    transplantation therapy and disease odeling. Despite efforts to design hydrogels
    as cell culture platforms for in vitro vascularization, development of vascularized
    tissues recapitulating the natural structures and functions remains difficult
    due to a poor understanding of the relationships between the matrix microstructures
    and tube formation of endothelial cells. Herein, we developed microfiber network
    hydrogels with microporous structures by controlling the liquid−liquid phase separation
    (LLPS) of proteins and matrix structures in hydrogels. Extracellular matrix protein
    gelatin was modified with hydrogen-bonding moieties and mixed with hyaluronic
    acid sodium salt to form microfiber network structures. Gelatin gelation and hyaluronic
    acid sodium salt dissolution led to the formation of a microporous microfiber
    network hydrogel formation. Matrix structures of hydrogels were modified by controlling
    LLPS that affects endothelial cell tube formation. Vascularization was improved
    using laminin peptides and coculturing with mesenchymal stem cells. Overall, our
    approach exhibits the potential to induce in vitro vascularization for regenerative
    medicine and disease modeling applications.
  description_type: abstract
  lang: und

## Creator

- name: Akihiro Nishiguchi
  role: author
  orcid: https://orcid.org/0000-0002-3160-6385
- name: Erino Araki
  role: author
- name: Debabrata Palai
  role: author
  orcid: https://orcid.org/0000-0003-1192-6143
- name: Shima Ito
  role: author
  orcid: https://orcid.org/0000-0002-3233-617X
- name: Tetsushi Taguchi
  role: author
  orcid: https://orcid.org/0000-0003-2541-2530

## Contact agent



## Publisher

organization: American Chemical Society (ACS)

## Managing organization



## Keyword

- subject: Regenerative medicine, Hydrogel, Liquid-liquid phase separation, Porous
    material
  schema: not_defined

## Rights

- description: This document is the unedited Author’s version of a Submitted Work
    that was subsequently accepted for publication in American Chemical Society, copyright
    © 2024 American Chemical Society after peer review. To access the final edited
    and published work see https://doi.org/10.1021/acs.biomac.4c00836
  identifier: http://rightsstatements.org/vocab/InC/1.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Biomacromolecules
  issn: '15257797'
  volume: '25'
  issue: '9'
  start_page: 6146
  end_page: 6154

## Conference



## Related item



## Funding

- funder_name: Inamori Foundation
- identifier: 22H03962
  funder_name: Japan Society for the Promotion of Science
- identifier: 23H01718
  funder_name: Japan Society for the Promotion of Science
- funder_name: Uehara Memorial Foundation

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## Structural feature for specimen



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## Process for specimen treatment



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## Fileset

- id: a0a6d53a-7812-4e25-add2-095e21f18003
  filename: Manuscript-F.pdf
  content_type: application/pdf
  size: 1904140
  md5: 658a269de51b5c85ddbc8786da3b4b23

## Thumbnail

fileset_id: a0a6d53a-7812-4e25-add2-095e21f18003
filename: Manuscript-F.pdf