# Porous microwell scaffolds for 3D culture of pancreatic beta cells to promote cell aggregation and insulin secretion

https://mdr.nims.go.jp/datasets/4e49742b-b9e2-4e96-9d01-7bb48c86b4d5

## File

- [Porous microwell scaffolds for 3D culture of pancreatic beta cells to promote cell aggregation and insulin secretion.docx](https://mdr.nims.go.jp/filesets/ce60fb9b-16e5-486d-bff6-c6c81f279929/download) ([Detail](https://mdr.nims.go.jp/filesets/ce60fb9b-16e5-486d-bff6-c6c81f279929.md))

## Id

4e49742b-b9e2-4e96-9d01-7bb48c86b4d5

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-10-09T07:01:54.364979Z

## Updated at

2025-01-15T07:31:11.097627Z

## Published at

2025-01-15T07:31:11.264822Z

## Doi

https://doi.org/10.48505/nims.4831

## First published url

https://doi.org/10.1039/d3ma01048a

## Date published

2024-01-15

## Recorded date published

2024-3-4

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Porous microwell scaffolds for 3D culture of pancreatic beta cells to promote
    cell aggregation and insulin secretion
  title_type: original
  lang: en

## Description

- description: Malfunctioning of the pancreas leads to insufficient insulin secretion
    from pancreatic beta cells in type 1 diabetes mellitus patients. Even though transplantation
    of pancreatic beta cells is making progress in diabetic treatment, further promoting
    insulin secretion of pancreatic beta cells remains a challenge. In this study,
    gelatin–PLGA mesh based porous microwell scaffolds were prepared for three-dimensional
    (3D) culture of pancreatic beta cells to promote their aggregation and insulin
    secretion functions. The microwell structure was fabricated on a biodegradable
    polymer mesh by using an ice particulate template method. The size of microwells
    could be controlled by the dimension of ice particulates. The microwells had a
    concave morphology surrounded by dense ultra-small pores. RIN-5F cells cultured
    in the porous microwell scaffolds had high viability and formed grape-like aggregates.
    More importantly, insulin secretion of RIN-5F cells was enhanced by culturing
    in the porous microwell scaffolds compared to culturing in flat scaffold and normal
    cell culture plates. The results suggested that the porous microwell scaffolds
    promoted aggregation formation and insulin secretion of pancreatic beta cells.
    The porous microwell scaffolds hold high potential as a novel 3D culture model
    for diabetes mellitus.
  description_type: abstract
  lang: und

## Creator

- name: Huajian Chen
  role: author
  orcid: https://orcid.org/0000-0003-1643-9782
- name: Tianjiao Zeng
  role: author
  orcid: https://orcid.org/0000-0002-1286-0337
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26
- name: Toru Yoshitomi
  role: author
  orcid: https://orcid.org/0000-0003-3847-1812
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26
- name: Naoki Kawazoe
  role: author
  orcid: https://orcid.org/0000-0003-3916-0709
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26
- name: Hirotake Komatsu
  role: author
- name: Yingnan Yang
  role: author
- name: Guoping Chen
  role: author
  orcid: https://orcid.org/0000-0001-6753-3678
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26

## Contact agent



## Publisher

organization: Royal Society of Chemistry (RSC)

## Managing organization



## Keyword

- subject: Porous microwell scaffold
  schema: not_defined
- subject: Pancreatic beta cell
  schema: not_defined
- subject: Insulin secretion
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2024-01-15
end_date: 2025-01-15

## Journal

- title: Materials Advances
  issn: '26335409'
  volume: '5'
  issue: '5'
  start_page: 2019
  end_page: 2026

## Conference



## Related item



## Funding

- identifier: 19H04475
  funder_name: Japan Society for the Promotion of Science
- identifier: 21H03830
  funder_name: Japan Society for the Promotion of Science
- identifier: 22K19926
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



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## Fileset

- id: ce60fb9b-16e5-486d-bff6-c6c81f279929
  filename: Porous microwell scaffolds for 3D culture of pancreatic beta cells to
    promote cell aggregation and insulin secretion.docx
  content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
  size: 6303198
  md5: bb534aa43a234a9b64935f0e5af8f9e8

## Thumbnail

fileset_id: ce60fb9b-16e5-486d-bff6-c6c81f279929
filename: Porous microwell scaffolds for 3D culture of pancreatic beta cells to promote
  cell aggregation and insulin secretion.docx