# Concentrated Polymer Brush-Modified Magnetic Particles for a Diagnostic Immunoassay

https://mdr.nims.go.jp/datasets/47902b69-0556-4f08-85c8-0f052cc1278f

## Files

- [Langmuir 2025.pdf](https://mdr.nims.go.jp/filesets/5c93dd41-f2d7-489c-b9cd-0b9a66fdb49c/download) ([Detail](https://mdr.nims.go.jp/filesets/5c93dd41-f2d7-489c-b9cd-0b9a66fdb49c.md))

## Id

47902b69-0556-4f08-85c8-0f052cc1278f

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-12-12T10:25:42.346156Z

## Updated at

2025-12-15T01:31:43.237522Z

## Published at

2025-12-18T10:08:30.348644Z

## Doi



## First published url

https://doi.org/10.1021/acs.langmuir.5c04175

## Date published

2025-12-09

## Recorded date published

2025-12-9

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Concentrated Polymer Brush-Modified Magnetic Particles for a Diagnostic Immunoassay
  title_type: original
  lang: en

## Description

- description: Non-specific protein absorption is an ongoing problem in the development
    of highly sensitive magnetic particle (MP)-based diagnostic assay, whereby the
    MP surface undergoes biofouling - significantly reducing the limit of detection
    and sensitivity of the device. In this study, a bioinert, concentrated polymer
    brush (CPBs) composed of poly[poly(ethylene oxide) methyl ether methacrylate]
    (PPEGMA) was employed to reduce protein absorption onto the MP surfaces, to which
    an anti-human serum albumin antibody (Ab) was then immobilized onto the brush
    layer by click-chemistry to demonstrate its application in immunoassays. The amount
    of antibody grafted onto the ends of the brush coating was quantified by BCA assay
    with high grafting efficiency (~ 0.80 antibody/chain). Furthermore, when using
    the MPs with PPEGMA conjugated with Ab (MP-PPEGMA-Ab) as an immunoassay platform,
    we were able to determine the capture efficacy of human serum albumin (HSA) in
    both a buffered solution and diluted human serum by colorimetric analysis; and
    further confirmation via liquid chromatography/mass spectrometry – in which our
    MPs showed a high selectivity towards the targeted analyte. Lastly, we demonstrated
    the MP-PPEGMA-Ab was capable of detecting human serum albumin as low as 6.4 ng
    mL-1, 30% more sensitive than unmodified MP – demonstrating the impact of CPBs
    for diagnostic assays. Due to its high selectivity and sensitivity, our CPB-based
    MPs are expected to be applicable for a wide range of immunoassay applications
    by employing different bioinert polymers and biofunctional groups.
  description_type: abstract
  lang: und

## Creator

- name: Gabriel Tai Huynh
  role: author
- name: Jun Qiu
  role: author
- name: Edith van den Bosch
  role: author
- name: Tomohiko Yamazaki
  role: author
  orcid: https://orcid.org/0000-0003-2136-8042
- name: Chiaki Yoshikawa
  role: author
  orcid: https://orcid.org/0000-0002-6589-387X

## Contact agent



## Publisher

organization: American Chemical Society (ACS)

## Managing organization



## Keyword

- subject: magnetic particle
  schema: not_defined
- subject: concentrated polymer brush
  schema: not_defined
- subject: immunoassay
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin



## Embargo



## Journal

- title: Langmuir
  issn: '07437463'
  volume: '41'
  issue: '48'
  start_page: 32432
  end_page: 32442

## Conference



## Related item



## Funding

- identifier: 19KK0368
  funder_name: Japan Society for the Promotion of Science
- identifier: 22H02133
  funder_name: Japan Society for the Promotion of Science
- identifier: 24KF0166
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



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## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



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## Process for specimen treatment



## Computational method



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## Fileset

- id: 5c93dd41-f2d7-489c-b9cd-0b9a66fdb49c
  filename: Langmuir 2025.pdf
  content_type: application/pdf
  size: 5124822
  md5: 131a079db785a56e2e043189c9b863ee

## Thumbnail

fileset_id: 5c93dd41-f2d7-489c-b9cd-0b9a66fdb49c
filename: Langmuir 2025.pdf