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説明:

Non-specific protein absorption is an ongoing problem in the development of highly sensitive magnetic particle (MP)-based diagnostic assay, whereby the MP surface undergoes biofouling - significantly reducing the limit of detection and sensitivity of the device. In this study, a bioinert, concentrated polymer brush (CPBs) composed of poly[poly(ethylene oxide) methyl ether methacrylate] (PPEGMA) was employed to reduce protein absorption onto the MP surfaces, to which an anti-human serum albumin antibody (Ab) was then immobilized onto the brush layer by click-chemistry to demonstrate its application in immunoassays. The amount of antibody grafted onto the ends of the brush coating was quantified by BCA assay with high grafting efficiency (~ 0.80 antibody/chain). Furthermore, when using the MPs with PPEGMA conjugated with Ab (MP-PPEGMA-Ab) as an immunoassay platform, we were able to determine the capture efficacy of human serum albumin (HSA) in both a buffered solution and diluted human serum by colorimetric analysis; and further confirmation via liquid chromatography/mass spectrometry – in which our MPs showed a high selectivity towards the targeted analyte. Lastly, we demonstrated the MP-PPEGMA-Ab was capable of detecting human serum albumin as low as 6.4 ng mL-1, 30% more sensitive than unmodified MP – demonstrating the impact of CPBs for diagnostic assays. Due to its high selectivity and sensitivity, our CPB-based MPs are expected to be applicable for a wide range of immunoassay applications by employing different bioinert polymers and biofunctional groups.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: magnetic particle, concentrated polymer brush, immunoassay

刊行年月日: 2025-12-09

出版者: American Chemical Society (ACS)

掲載誌:

• Langmuir (ISSN: 07437463) vol. 41 issue. 48 p. 32432-32442

研究助成金:

• Japan Society for the Promotion of Science 19KK0368
• Japan Society for the Promotion of Science 22H02133
• Japan Society for the Promotion of Science 24KF0166

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.1021/acs.langmuir.5c04175

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更新時刻: 2025-12-15 10:31:43 +0900

MDRでの公開時刻: 2025-12-18 19:08:30 +0900