# Preparation and antibacterial activities of silver nanoparticle-loaded hydroxyapatite/collagen bone-like nanocomposite

https://mdr.nims.go.jp/datasets/42cfe2e9-d4d0-4249-a4c1-7bd8d7bf4711

## File

- [Ag-HAp_Col s41598-026-54878-2.pdf](https://mdr.nims.go.jp/filesets/2d5bc742-b948-468b-aabc-485627d5fb00/download) ([Detail](https://mdr.nims.go.jp/filesets/2d5bc742-b948-468b-aabc-485627d5fb00.md))
- [Ag-HAp_Col 41598_2026_54878_MOESM1_ESM.pdf](https://mdr.nims.go.jp/filesets/b740786c-5104-42ab-a3da-65fd85d2903d/download) ([Detail](https://mdr.nims.go.jp/filesets/b740786c-5104-42ab-a3da-65fd85d2903d.md))

## Id

42cfe2e9-d4d0-4249-a4c1-7bd8d7bf4711

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2026-08-19T00:36:52.523916Z

## Updated at

2026-08-19T04:49:18.782622Z

## Published at

2026-08-19T07:24:47.838497Z

## Doi



## First published url

https://doi.org/10.1038/s41598-026-54878-2

## Date published

2026-06-01

## Recorded date published



## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Preparation and antibacterial activities of silver nanoparticle-loaded hydroxyapatite/collagen
    bone-like nanocomposite
  title_type: original
  lang: en

## Description

- description: A hydroxyapatite/collagen bone-like nanocomposite (HAp/Col) loaded
    with silver nanoparticles (AgNPs) was prepared by immersion of HAp/Col powder
    in the AgNPs solution. The adsorption-desorption properties on the preparation
    process were investigated, followed by the evaluation of antibacterial activities
    and cytotoxicity. Adsorption of AgNPs on HAp/Col powder was almost finished at
    240 min after mixing. Adsorption isotherm revealed that maximum adsorption amount
    was 1621.9 mg/g with the best fitted model, Dubinin-Radushkevich adsorption model.
    The AgNPs-loaded HAp/Col membrane prepared by immersion into 1.6 µg/mL and 6.3
    µg/mL AgNPs colloidal solution demonstrated no survival of Escherichia coli and
    Staphylococcus aureus, respectively. Using human osteoblastic cell line, MG-63,
    no significant cytotoxicity was observed for the AgNPs-loaded HAp/Col membrane
    prepared with 6.3-12.5 µg/mL AgNPs colloidal solution. These results suggested
    that AgNPs-loaded HAp/Col would be a good candidate for antibacterial bone void
    fillers and coating materials for orthopedic and dental implants.
  description_type: abstract
  lang: eng

## Creator

- name: Masanori Kikuchi
  role: author
  orcid: https://orcid.org/0000-0002-9451-8147
  organization: National Institute for Materials Science
  department: Research Center for Macromolecules and Biomaterials/Biomaterials Field/Bioceramics
    Group
- name: Taku Yoshida
  role: author
  organization: Faculty of IST, Hokkaido University
- name: Yasushi Suetsugu
  role: author
  orcid: https://orcid.org/0000-0002-8161-1908
  organization: National Institute for Materials Science
  department: Research Center for Macromolecules and Biomaterials/Biomaterials Field/Bioceramics
    Group
- name: Akiko Yamamoto
  role: author
  orcid: https://orcid.org/0000-0002-9182-4886
  organization: National Institute for Materials Science
  department: Research Center for Macromolecules and Biomaterials

## Contact agent



## Publisher

organization: Springer Science and Business Media LLC

## Managing organization



## Keyword

- subject: Hydroxyapatite/collagen bone-like nanocomposite
  schema: not_defined
- subject: Silver nanoparticles
  schema: not_defined
- subject: Anti-microbial
  schema: not_defined
- subject: Cytotoxicity
  schema: not_defined
- subject: Bone void filler
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Scientific Reports
  issn: '20452322'
  volume: '16'
  issue: '1'

## Conference



## Related item



## Funding

- identifier: 21im0210221h0203
  funder_name: Japan Agency for Medical Research and Development

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 2d5bc742-b948-468b-aabc-485627d5fb00
  filename: Ag-HAp_Col s41598-026-54878-2.pdf
  content_type: application/pdf
  size: 2109762
  md5: 53224532e91a81c941a0b6c4dd631cb1
- id: b740786c-5104-42ab-a3da-65fd85d2903d
  filename: Ag-HAp_Col 41598_2026_54878_MOESM1_ESM.pdf
  content_type: application/pdf
  size: 430317
  md5: 252596edbe1e4f32487b4bea6ac8ce13

## Thumbnail

fileset_id: 2d5bc742-b948-468b-aabc-485627d5fb00
filename: Ag-HAp_Col s41598-026-54878-2.pdf