# Rapid isolation of extracellular vesicles from stem cell conditioned medium using osmosis-driven filtration

https://mdr.nims.go.jp/datasets/3b4618d9-3385-47d7-8aa4-e53d33589f82

## File

- [Rapid isolation of extracellular vesicles from stem cell conditioned medium using osmosis-driven filtration.pdf](https://mdr.nims.go.jp/filesets/a43aba69-d2fc-4518-9e0c-00283d6f2bb9/download) ([Detail](https://mdr.nims.go.jp/filesets/a43aba69-d2fc-4518-9e0c-00283d6f2bb9.md))

## Id

3b4618d9-3385-47d7-8aa4-e53d33589f82

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-04-16T08:11:36.678104Z

## Updated at

2025-07-16T07:14:59.674010Z

## Published at

2025-04-20T08:17:32.691004Z

## Doi

https://doi.org/10.48505/nims.5437

## First published url

https://doi.org/10.1080/14686996.2025.2485668

## Date published

2025-12-31

## Recorded date published

2025-12-31

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Rapid isolation of extracellular vesicles from stem cell conditioned medium
    using osmosis-driven filtration
  title_type: original
  lang: en

## Description

- description: Extracellular vesicles (EVs) hold significant promise as biomarkers
    and therapeutics, yet their isolation remains challenging due to their low abundance
    and complex sample matrices. Here, we introduce EV-Osmoprocessor (EVOs), a novel
    device that leverages osmosis-driven filtration for rapid and efficient EV isolation.
    EVOs employs a high osmolarity polymer solution to concentrate EVs while simultaneously
    removing smaller contaminants. Compared to traditional methods such as ultracentrifugation
    and precipitation, EVOs offers speed and convenience, achieving a 50-fold volume
    reduction in under 2 h. Our results show that EVOs retained EVs and removed >99%
    albumin from the cell conditioned culture medium (CCM). The isolated EVs exhibited
    a particle size distribution centered around 140 nm, which was very similar to
    EVs isolated via precipitation or ultracentrifugation. The standalone EVOs process
    achieved a particle:protein ratio (EV purity) of ~107 particles/µg protein. Comprehensive
    characterization, including cryo-electron microscopy, validation of protein markers
    and known miRNA cargo confirmed the successful isolation of EVs. Functional assays,
    based on protection of cardiomyocytes from hypoxia/reoxygenation injury, demonstrated
    the bioactivity of EVOs-isolated EVs. Furthermore, we show that EVOs can be used
    to concentrate 30 ml of CCM into a 0.5 ml solution, which was then further processed
    with size-exclusion chromatography (SEC), improving EV purity to ~109 particles/µg
    protein. This work establishes EVOs as a promising tool for EV research and clinical
    applications, offering a streamlined approach to EV isolation with enhanced analytical
    performance.
  description_type: abstract
  lang: en

## Creator

- name: Casey Y. Huang
  role: author
  organization: National Taiwan University of Science and Technology
  department: a Department of Material Science and Engineering
- name: Helen Nguyen
  role: author
- name: David J. Lundy
  role: author
- name: James. J Lai
  role: author

## Contact agent



## Publisher

organization: Taylor & Francis

## Managing organization



## Keyword

- subject: Exosome
  schema: not_defined
- subject: mesenchymal stromal cell
  schema: not_defined
- subject: cardiomyocyte
  schema: not_defined
- subject: bioprocessing
  schema: not_defined
- subject: polymer
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Science and Technology of Advanced Materials
  issn: '14686996'
  volume: '26'
  issue: '1'
  article_number: '2485668'

## Conference



## Related item



## Funding



## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: a43aba69-d2fc-4518-9e0c-00283d6f2bb9
  filename: Rapid isolation of extracellular vesicles from stem cell conditioned medium
    using osmosis-driven filtration.pdf
  content_type: application/pdf
  size: 3654659
  md5: 944ee065398876e60441d92d81980f52

## Thumbnail

fileset_id: a43aba69-d2fc-4518-9e0c-00283d6f2bb9
filename: Rapid isolation of extracellular vesicles from stem cell conditioned medium
  using osmosis-driven filtration.pdf