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The mechanical properties of the cellular microenvironment contribute significantly to cell behavior. Thus, deformable phospholipid-decorated perfluorocarbon interfaces have emerged for further expansion of material mechanics to an ultimate soft range as cell scaffolds. In addition, a highly deformable state requires the material to be robust enough to adapt to dynamic cellular forces. However, the effect of interfacial viscosity on the cell adhesion behavior and material robustness remains unknown on the super-soft substrate. To address these issues, an interfacial phospholipid membrane (IPLM) with tunable viscosity is constructed by varying the mixing ratio of saturated and unsaturated lipid layers. By co-assembling a cell adhesive and fluorescent lipid into the IPLM, it is shown that higher viscosity interfaces with lower unsaturated lipid content are preferred from the viewpoint of cell spreading. However, a viscosity that is too high for 0% unsaturated lipid alters the lipid layer to a brittle solid-like nature, making it less adaptive to cell traction-induced high deformation. Therefore, at least a trace amount of unsaturated lipids is required to maintain the robustness of fluid scaffolds. These findings are useful for the design of biomimetic materials and the long-term investigation of cell-matrix mechanical interactions in highly adaptive environments.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: Mechanobiology, Fluid scaffold, Viscosity, Interface

刊行年月日: 2025-08-22

出版者: Wiley

掲載誌:

• Advanced NanoBiomed Research (ISSN: 26999307) vol. 5 issue. 11 2500076

研究助成金:

• Japan Society for the Promotion of Science 23K17481
• Japan Society for the Promotion of Science 22H00596

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.1002/anbr.202500076

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更新時刻: 2025-12-09 16:31:00 +0900

MDRでの公開時刻: 2025-12-09 16:26:05 +0900