# Influence of viscosity on adipogenic and osteogenic differentiation of mesenchymal stem cells during 2D culture

https://mdr.nims.go.jp/datasets/35e0e921-bb87-41a8-b31f-ffc1546b63d6

## File

- [Influence of viscosity on adipogenic and osteogenic differentiation of mesenchymal stem cells during 2D culture.pdf](https://mdr.nims.go.jp/filesets/1ffb257f-ad36-434a-85d3-2d8e71a9c388/download) ([Detail](https://mdr.nims.go.jp/filesets/1ffb257f-ad36-434a-85d3-2d8e71a9c388.md))

## Id

35e0e921-bb87-41a8-b31f-ffc1546b63d6

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-10-25T01:30:17.136444Z

## Updated at

2025-09-23T23:30:19.201339Z

## Published at

2025-09-23T23:23:28.685071Z

## Doi

https://doi.org/10.48505/nims.4897

## First published url

https://doi.org/10.1039/d4bm00710g

## Date published

2024-09-24

## Recorded date published

2024-10-22

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Influence of viscosity on adipogenic and osteogenic differentiation of mesenchymal
    stem cells during 2D culture
  title_type: original
  lang: en

## Description

- description: 'In this study, the influence of viscosity on the adipogenic and osteogenic
    differentiation of human mesenchymal stem cells (hMSCs) was investigated by adjusting
    the viscosity of the culture medium. Viscosity exhibited different effects on
    adipogenic and osteogenic differentiation of hMSCs during two-dimensional (2D)
    culture. High viscosity facilitated osteogenic while inhibiting adipogenic differentiation.
    During adipogenic differentiation, the effect of viscosity on cell proliferation
    was negligible. However, during osteogenic differentiation, high viscosity decreased
    cell proliferation. The different influence of viscosity could be explained by
    the activation of mechanotransduction regulators of Yes-associated protein (YAP)
    and β-catenin. High viscosity could promote YAP and β-catenin nuclear translocation
    during osteogenic differentiation, which was responsible for the increased osteogenesis.
    High viscosity inhibited adipogenesis through promoting YAP nuclear translocation. '
  description_type: abstract
  lang: und

## Creator

- name: Chengyu Lu
  role: author
  orcid: https://orcid.org/0000-0002-8334-3177
- name: Tianjiao Zeng
  role: author
  orcid: https://orcid.org/0000-0002-1286-0337
- name: Man Wang
  role: author
- name: Toru Yoshitomi
  role: author
  orcid: https://orcid.org/0000-0003-3847-1812
- name: Naoki Kawazoe
  role: author
  orcid: https://orcid.org/0000-0003-3916-0709
- name: Yingnan Yang
  role: author
- name: Guoping Chen
  role: author
  orcid: https://orcid.org/0000-0001-6753-3678

## Contact agent



## Publisher

organization: Royal Society of Chemistry (RSC)

## Managing organization



## Keyword

- subject: viscosity
  schema: not_defined
- subject: adipogenic differentiation
  schema: not_defined
- subject: osteogenic differentiation
  schema: not_defined
- subject: mesenchymal stem cells
  schema: not_defined

## Rights

- identifier: http://rightsstatements.org/vocab/InC/1.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2024-09-24
end_date: 2025-09-24

## Journal

- title: Biomaterials Science
  issn: '20474830'
  volume: '12'
  issue: '21'
  start_page: 5598
  end_page: 5609

## Conference



## Related item



## Funding

- identifier: 21H03830
  funder_name: Japan Society for the Promotion of Science
- identifier: 22K19926
  funder_name: Japan Society for the Promotion of Science
- identifier: 24K03289
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



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## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Fileset

- id: 1ffb257f-ad36-434a-85d3-2d8e71a9c388
  filename: Influence of viscosity on adipogenic and osteogenic differentiation of
    mesenchymal stem cells during 2D culture.pdf
  content_type: application/pdf
  size: 2132316
  md5: cd6098fe906636efd92cef2106687847

## Thumbnail

fileset_id: 1ffb257f-ad36-434a-85d3-2d8e71a9c388
filename: Influence of viscosity on adipogenic and osteogenic differentiation of mesenchymal
  stem cells during 2D culture.pdf