Download file (2.03 MB)
Download as zip (2 MB)

Collection

Citation

Description:

In this study, the influence of viscosity on the adipogenic and osteogenic differentiation of human mesenchymal stem cells (hMSCs) was investigated by adjusting the viscosity of the culture medium. Viscosity exhibited different effects on adipogenic and osteogenic differentiation of hMSCs during two-dimensional (2D) culture. High viscosity facilitated osteogenic while inhibiting adipogenic differentiation. During adipogenic differentiation, the effect of viscosity on cell proliferation was negligible. However, during osteogenic differentiation, high viscosity decreased cell proliferation. The different influence of viscosity could be explained by the activation of mechanotransduction regulators of Yes-associated protein (YAP) and β-catenin. High viscosity could promote YAP and β-catenin nuclear translocation during osteogenic differentiation, which was responsible for the increased osteogenesis. High viscosity inhibited adipogenesis through promoting YAP nuclear translocation.

Rights:

• In Copyright
  

Keyword: viscosity, adipogenic differentiation, osteogenic differentiation, mesenchymal stem cells

Date published: 2024-09-24

Publisher: Royal Society of Chemistry (RSC)

Journal:

• Biomaterials Science (ISSN: 20474830) vol. 12 issue. 21 p. 5598-5609

Funding:

• Japan Society for the Promotion of Science 21H03830
• Japan Society for the Promotion of Science 22K19926
• Japan Society for the Promotion of Science 24K03289

Manuscript type: Author's version (Accepted manuscript)

MDR DOI: https://doi.org/10.48505/nims.4897

First published URL: https://doi.org/10.1039/d4bm00710g

Related item:

Other identifier(s):

Contact agent:

Updated at: 2025-09-24 08:30:19 +0900

Published on MDR: 2025-09-24 08:23:28 +0900