# Scalable liposomes functionalization via membrane lipid exchange mechanisms https://mdr.nims.go.jp/datasets/35c97b66-810d-4e63-9107-c444b9247908 ## File - [MDR_Manuscript_liposome-induced membrane exchange_Nano Today (1).docx](https://mdr.nims.go.jp/filesets/1f671512-eea9-41f3-9f8d-2b6fa31f4958/download) ([Detail](https://mdr.nims.go.jp/filesets/1f671512-eea9-41f3-9f8d-2b6fa31f4958.md)) - [MDR_SI_liposome-induced membrane exchange_Nano Today.docx](https://mdr.nims.go.jp/filesets/7184bcad-0c16-4d36-a41e-63123b74e511/download) ([Detail](https://mdr.nims.go.jp/filesets/7184bcad-0c16-4d36-a41e-63123b74e511.md)) ## Id 35c97b66-810d-4e63-9107-c444b9247908 ## Local identifier ## Visibility open_to_public ## State published ## Created at 2026-01-18T23:03:24.736730Z ## Updated at 2026-01-19T00:54:02.209165Z ## Published at 2026-01-19T03:21:42.041209Z ## Doi https://doi.org/10.48505/nims.6141 ## First published url https://doi.org/10.1016/j.nantod.2025.102630 ## Date published 2025-01-10 ## Recorded date published 2025-4 ## Resource type journal_article ## Manuscript type accepted_manuscript ## Collection ## Title - title: Scalable liposomes functionalization via membrane lipid exchange mechanisms title_type: original lang: en ## Description - description: "Extracellular vesicles are pivotal in intercellular communication and hold significant promise for medical applications. However, limitations in their mass production and challenges in replicating their complex functions with artificial liposomes necessitate innovative solutions. We functionalize liposomes by combining the scalable production advantages of artificial liposomes with the vesicle fusion and formation mechanisms of bacteria. By incubating the gram-negative Shewanella oneidensis MR-1, known for its electrochemically active outer membrane cytochromes (OMCs), with liposomes containing 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine for 24 hours, we achieved a substantial yield of membrane-integrated liposomes (MILs) incorporating OMCs. Circular dichroism spectroscopy confirmed the preservation of redox activity and strong inter-heme exciton coupling in the OMCs. These components were successfully delivered to Escherichia coli K-12 by incubation with MILs, retaining their functionality. Furthermore, the slow membrane exchange process did not result in cellular viability loss or lysis, allowing for the recycling of microbial cells and minimizing contaminants from lysed cells, which is advantageous for scaling up. Building on our previous work where MIL-coated titanium dioxide nanoparticles significantly enhanced radical production and effectively treated orthotopic liver tumors in vivo, our methodology to generate the MIL has promising potential to spearhead novel integrations of synthetic and biological systems for medical technologies.\r\n" description_type: abstract lang: und ## Creator - name: Xizi Long role: author organization: National Institute for Materials Science - name: Chiho Kataoka-Hamai role: author orcid: https://orcid.org/0000-0002-4068-0405 organization: National Institute for Materials Science - name: Chia-Lun Ho role: author organization: National Institute for Materials Science - name: Wei-Lun Huang role: author - name: Yi-Ho Kuo role: author - name: Li-Ting Yang role: author - name: Wei-Peng Li role: author organization: National Institute for Materials Science - name: Akihiro Okamoto role: author orcid: https://orcid.org/0000-0002-8102-4316 organization: National Institute for Materials Science ## Contact agent ## Publisher organization: Elsevier BV ## Managing organization ## Keyword - subject: liposome schema: not_defined - subject: outer-membrane vesicles schema: not_defined - subject: extracellular electron transport schema: not_defined - subject: cytochrome schema: not_defined ## Rights - identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/ ## Other identifier(s) ## Data origin - data_origin_type: other ## Embargo start_date: 2025-01-10 end_date: 2026-01-10 ## Journal - title: Nano Today issn: '17480132' volume: '61' article_number: '102630' ## Conference ## Related item ## Funding - identifier: 17H04969 funder_name: Japan Society for the Promotion of Science - identifier: JPMJPR19H1 funder_name: Precursory Research for Embryonic Science and Technology - identifier: P20105 funder_name: Precursory Research for Embryonic Science and Technology - identifier: MOE-109-YSFMS-1019-001-P1 funder_name: Ministry of Education - identifier: 19gm6010002h0004 funder_name: Japan Agency for Medical Research and Development - identifier: 2024JJ4035 funder_name: Natural Science Foundation of Hunan Province - funder_name: National Science and Technology Council - funder_name: National Cheng Kung University - funder_name: Japan Science and Technology Agency - identifier: 112-2113-M-037-014-MY2 funder_name: National Science and Technology Council - identifier: 113-2320-B-037-007- funder_name: National Science and Technology Council ## Instrument ## Instrument operator ## Instrument managing organization ## Measurement method ## Specimen ## Chemical composition ## Structure for specimen ## Structural feature for specimen ## Specific property for specimen ## Process for specimen treatment ## Computational method ## Energy level/transition state ## Software ## Custom property ## Fileset - id: 1f671512-eea9-41f3-9f8d-2b6fa31f4958 filename: MDR_Manuscript_liposome-induced membrane exchange_Nano Today (1).docx content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document size: 3237203 md5: 9c13cef6e8f81eaa5dcf18e542f98925 - id: 7184bcad-0c16-4d36-a41e-63123b74e511 filename: MDR_SI_liposome-induced membrane exchange_Nano Today.docx content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document size: 4189218 md5: df02bd85c0dcaba5920c07acfefa3098 ## Thumbnail fileset_id: 1f671512-eea9-41f3-9f8d-2b6fa31f4958 filename: MDR_Manuscript_liposome-induced membrane exchange_Nano Today (1).docx