# Potential of Enzymatically Synthesized Hemozoin Analog as Th1 Cell Adjuvant

https://mdr.nims.go.jp/datasets/3034f9d2-7ee2-48ea-96f6-2fedba69c76c

## File

- [Potential of Enzymatically Synthesi-2024-Hoshi.pdf](https://mdr.nims.go.jp/filesets/460a8432-9277-4ff7-8b5f-d9ae9210a7b3/download) ([Detail](https://mdr.nims.go.jp/filesets/460a8432-9277-4ff7-8b5f-d9ae9210a7b3.md))

## Id

3034f9d2-7ee2-48ea-96f6-2fedba69c76c

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-10-03T23:41:10.979165Z

## Updated at

2024-10-04T03:30:16.983622Z

## Published at

2024-10-04T03:30:18.195252Z

## Doi



## First published url

https://doi.org/10.3390/nano14171440

## Date published

2024-09-03

## Recorded date published



## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Potential of Enzymatically Synthesized Hemozoin Analog as Th1 Cell Adjuvant
  title_type: original
  lang: en

## Description

- description: Hemozoin (Hz) is a heme crystal produced during malaria infection that
    stimulates immune cells, leading to the production of cytokines and chemokines.
    The immunostimulatory action of Hz has previously been applied in the development
    of alternative adjuvants. Crystallization of hemin is a chemical approach for
    producing Hz. Here, we focused on an enzymatic production method for Hz using
    the heme detoxification protein (HDP), which catalyzes heme dimer formation from
    hemin in Plasmodium. We examined the immunostimulatory effects of an enzymatically
    synthesized analog of Hz (esHz) produced by recombinant Plasmodium falciparum
    HDP. Enzymatically synthesized Hz stimulates a macrophage cell line and human
    peripheral mononuclear cells, leading to the production of interleukin (IL)-6
    and IL-12p40. In mice, subcutaneous administration of esHz together with an antigen,
    ovalbumin (OVA), increased the OVA-specific immunoglobulin (Ig) G2c isotype level
    in the serum, whereas OVA-specific IgG1 was not induced. Our findings suggest
    that esHz is a useful Th-1 cell adjuvant.
  description_type: abstract
  lang: und

## Creator

- name: Kazuaki Hoshi
  role: author
- name: Anh Thi Tram Tu
  role: author
- name: Miwako Shobo
  role: author
- name: Karin Kettisen
  role: author
- name: Lei Ye
  role: author
  orcid: https://orcid.org/0000-0002-3646-4072
- name: Leif Bülow
  role: author
  orcid: https://orcid.org/0000-0003-4966-8610
- name: Yoji Hakamata
  role: author
  orcid: https://orcid.org/0000-0002-0138-6692
- name: Tetsuya Furuya
  role: author
  orcid: https://orcid.org/0000-0003-3979-7072
- name: Ryutaro Asano
  role: author
  orcid: https://orcid.org/0000-0001-6795-8377
- name: Wakako Tsugawa
  role: author
  orcid: https://orcid.org/0000-0001-6299-5267
- name: Kazunori Ikebukuro
  role: author
  orcid: https://orcid.org/0000-0003-2838-0562
- name: Koji Sode
  role: author
  orcid: https://orcid.org/0000-0002-9833-2091
- name: Tomohiko Yamazaki
  role: author
  orcid: https://orcid.org/0000-0003-2136-8042

## Contact agent



## Publisher

organization: MDPI AG

## Managing organization



## Keyword

- subject: hemozoin analog
  schema: not_defined
- subject: heme detoxification protein
  schema: not_defined
- subject: adjuvant
  schema: not_defined
- subject: vaccine
  schema: not_defined
- subject: Th-1 immunity
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Nanomaterials
  issn: '20794991'
  volume: '14'
  issue: '17'
  article_number: '1440'

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## Related item



## Funding

- identifier: 17KK0122
  funder_name: Japan Society for the Promotion of Science Kakenhi
  description: 植物・微生物由来ヘミンからの免疫活性化ヘムポリマーの合成
- identifier: 15K06589
  funder_name: Japan Society for the Promotion of Science Kakenhi
  description: ヘミン誘導体から構成される免疫活性化剤の開発
- identifier: A18-58
  funder_name: AMED, Clinical research core institutes under the Tsukuba Clinical
    Research & Development Organization
  description: "AMED 橋渡し研究戦略的推進プログラム\r\nアジュバントとして機能する酵素合成ヘモゾインナノ粒⼦の開発"

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## Fileset

- id: 460a8432-9277-4ff7-8b5f-d9ae9210a7b3
  filename: Potential of Enzymatically Synthesi-2024-Hoshi.pdf
  content_type: application/pdf
  size: 2765355
  md5: e3564ae4bc4d74c0a6ed00a1688822a6

## Thumbnail

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filename: Potential of Enzymatically Synthesi-2024-Hoshi.pdf