# Significance of homogeneous operation in light-assisted fixed-bed bioprocess under ammonia stress: Optimization, long-term operation and metagenomic analysis

https://mdr.nims.go.jp/datasets/2c556920-9397-47ae-916e-763ddfe7e4ff

## File

- [BITE-D-24-00471.pdf](https://mdr.nims.go.jp/filesets/659ac9f1-9699-4ac8-b5bf-f8ac886a51be/download) ([Detail](https://mdr.nims.go.jp/filesets/659ac9f1-9699-4ac8-b5bf-f8ac886a51be.md))

## Id

2c556920-9397-47ae-916e-763ddfe7e4ff

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-10-25T06:00:06.826611Z

## Updated at

2026-03-11T07:30:07.852268Z

## Published at

2026-03-11T05:05:57.790532Z

## Doi

https://doi.org/10.48505/nims.4898

## First published url

https://doi.org/10.1016/j.biortech.2024.130568

## Date published

2024-03-11

## Recorded date published

2024-5

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: 'Significance of homogeneous operation in light-assisted fixed-bed bioprocess
    under ammonia stress: Optimization, long-term operation and metagenomic analysis'
  title_type: original
  lang: en

## Description

- description: Activating microbes with light is a promising strategy for addressing
    ammonia-stressed anaerobic digestion (AD). However, as a critical in-process parameter,
    homogenous operation, in light-assisted AD amended by bio-fixed bed has received
    limited attention. This research endeavors to establish a uniform-illuminated
    biosystem and assess its practical feasibility through a 90-day semi-continuous
    operation at pilot scale under solar light illumination. With optimal stirring
    mode (intermittent stirring for 3 min every 15 min), robust methane yields were
    achieved across various organic loads, reaching 88.7–94.3% of theoretical yield
    under high ammonium stress (3500 mg/L). The metagenomic analysis unveiled that
    uniform illumination triggered synergistic effects in AD, fostering a diversified
    microbial consortium, enhancing carbohydrate and methane metabolism, and facilitating
    the formation of an electroactive bio-cluster. This study underscores the significance
    of homogenous illumination in AD systems for efficient waste-to-energy conversion,
    highlighting the implementation of solar light as a greener approach for scale-up
    application.
  description_type: abstract
  lang: eng

## Creator

- name: Yunxin Zhu
  role: author
  organization: School of Life and Environmental Sciences Degree Programs in Life
    and Earth Sciences Faculty of Life and Environmental Sciences, University of Tsukuba
- name: YuJia Chen
  role: author
  organization: School of Life and Environmental Sciences Degree Programs in Life
    and Earth Sciences Faculty of Life and Environmental Sciences, University of Tsukuba
- name: Guangqi An
  role: author
  organization: National Institute for Materials Science
  department: Research Center for Macromolecules and Biomaterials/Biomaterials Field/Tissue
    Regeneration Materials Group
  ror: https://ror.org/026v1ze26
- name: Cheng Zhang
  role: author
  organization: School of Life and Environmental Sciences Degree Programs in Life
    and Earth Sciences Faculty of Life and Environmental Sciences, University of Tsukuba
- name: Jingwei Yang
  role: author
  organization: School of Life and Environmental Sciences Degree Programs in Life
    and Earth Sciences Faculty of Life and Environmental Sciences, University of Tsukuba
- name: Rongyong Yang
  role: author
  organization: Shanghai High Victory Science and Technology Co., Ltd.
- name: Guoping Chen
  role: author
  orcid: https://orcid.org/0000-0001-6753-3678
  organization: National Institute for Materials Science
  department: Research Center for Macromolecules and Biomaterials/Biomaterials Field/Tissue
    Regeneration Materials Group
  ror: https://ror.org/026v1ze26
- name: Yingnan Yang
  role: author
  organization: School of Life and Environmental Sciences Degree Programs in Life
    and Earth Sciences Faculty of Life and Environmental Sciences, University of Tsukuba

## Contact agent



## Publisher

organization: Elsevier

## Managing organization



## Keyword

- subject: Light-assisted anaerobic digestion (AD), Ammonia inhibition, Homogenous
    operation, Pilot-scale evaluation, Bioinformatics analysis
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2024-03-11
end_date: 2026-03-12

## Journal

- title: BIORESOURCE TECHNOLOGY
  issn: '09608524'
  volume: '399'
  article_number: '130568'

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## Fileset

- id: 659ac9f1-9699-4ac8-b5bf-f8ac886a51be
  filename: BITE-D-24-00471.pdf
  content_type: application/pdf
  size: 2901537
  md5: 240b28c7374885572ae54875af40e881

## Thumbnail

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filename: BITE-D-24-00471.pdf