# Cell-Surface Inter-Cytochrome Electron Transfer Limits Biofilm Electron Conduction Kinetics in Shewanella oneidensis
https://mdr.nims.go.jp/datasets/2bdd3031-70c6-4859-afe2-f4b8011eb679
## File
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## Id
2bdd3031-70c6-4859-afe2-f4b8011eb679
## Local identifier
## Visibility
open_to_public
## State
published
## Created at
2026-01-18T23:09:48.842357Z
## Updated at
2026-01-19T01:00:13.824258Z
## Published at
2026-01-19T03:21:41.026563Z
## Doi
## First published url
https://doi.org/10.1021/jacs.5c10357
## Date published
2025-12-24
## Recorded date published
2025-12-24
## Resource type
journal_article
## Manuscript type
vor
## Collection
## Title
- title: |-
Cell-Surface Inter-Cytochrome Electron Transfer Limits Biofilm Electron Conduction Kinetics in
Shewanella oneidensis
title_type: original
lang: en
## Description
- description: The electrical conductivity of biofilms plays a critical role in advancing
bioelectronics for energy and environmental appli-cations, yet the underlying
mechanisms remain poorly under-stood. Previous studies proposed interheme electron
transfer between hemes 5 and 10 in the outer-membrane deca-heme cytochrome (OMC)
MtrC as the rate-limiting step in the bio-film electron conduction of Shewanella
oneidensis MR-1. However, the strong interheme electron coupling in MtrC sug-gests
that interprotein interactions may represent the primary barrier to biofilm electron
conduction. Here, we investigated biofilm electron conduction mechanism with a
focus on inter-protein electron transfer in S. oneidensis MR-1. Conductive currents
and their temperature dependence were measured for estimating the thermal activation
energy (Ea) using indium tin-doped oxide (ITO) interdigitated electrodes in wild-type
and mutant biofilms. While deletion of periplasmic cytochromes had negligible
impact on Ea, the deletion of OmcA or MtrC increased Ea threefold, revealing that
interprotein interactions, particularly between OmcA and MtrC, dominate biofilm
elec-tron transfer over clonal OMC interactions. Furthermore, sup-pressing outer-membrane
fluidity dramatically increased Ea, while inter-heme exciton coupling negligibly
changed in OMCs, confirming the critical role of protein diffusion and collision
on the outer membrane. Flavin binding to OmcA or MtrC reduced conduction currents
attributable to heme centers but enhanced those assignable to non-covalently bound
fla-vins, suggesting that flavin occupancy blocks hemes 2 and 7, which serve as
key interprotein electron transfer sites. These findings provide a mechanistic
foundation for engineering highly conductive biofilms through targeted protein
interface optimization, offering new avenues for the development of bioelectronic
technologies.
description_type: abstract
lang: und
## Creator
- name: Xinxin Wen
role: author
orcid: https://orcid.org/0000-0002-7762-9654
organization: National Institute for Materials Science
- name: Xizi Long
role: author
organization: National Institute for Materials Science
- name: Wenyuan Huang
role: author
orcid: https://orcid.org/0000-0002-5948-5618
organization: National Institute for Materials Science
- name: Masahiro Kuramochi
role: author
- name: Akihiro Okamoto
role: author
orcid: https://orcid.org/0000-0002-8102-4316
organization: National Institute for Materials Science
## Contact agent
## Publisher
organization: American Chemical Society (ACS)
## Managing organization
## Keyword
- subject: biofilm
schema: not_defined
- subject: thermal activation energy
schema: not_defined
- subject: extracellular electron transport
schema: not_defined
- subject: deca-heme cytochrome
schema: not_defined
- subject: circular dichroism spectroscopy
schema: not_defined
## Rights
- identifier: https://creativecommons.org/licenses/by/4.0/
date_licensed: 2025-12-15
## Other identifier(s)
## Data origin
## Embargo
## Journal
- title: Journal of the American Chemical Society
issn: '15205126'
volume: '147'
issue: '51'
start_page: 46932
end_page: 46940
## Conference
## Related item
## Funding
- identifier: 2024JJ4035
funder_name: Natural Science Foundation of Hunan Province
- identifier: JPMJPR19H1
funder_name: Precursory Research for Embryonic Science and Technology
- identifier: 19gm6010002h0004
funder_name: Japan Agency for Medical Research and Development
- identifier: 17H04969
funder_name: Japan Society for the Promotion of Science
- identifier: '42207073'
funder_name: National Natural Science Foundation of China
- identifier: 2024RC3210
funder_name: Huxiang Talent Foundation of Hunan Province
- identifier: HBZ20240138
funder_name: Hunan Natural Resources Research Post-Subsidy
## Instrument
## Instrument operator
## Instrument managing organization
## Measurement method
## Specimen
## Chemical composition
## Structure for specimen
## Structural feature for specimen
## Specific property for specimen
## Process for specimen treatment
## Computational method
## Energy level/transition state
## Software
## Custom property
## Fileset
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filename: Xinxin Long 2025 JACS cell-surface-inter-cytochrome-electron-transfer-limits-biofilm-electron-conduction-kinetics-in-shewanella-oneidensis.pdf
content_type: application/pdf
size: 5339336
md5: 250839697e0053d3dd66a6fae3df763b
- id: f2cd5f81-adc2-4fdb-a20a-b7517f651506
filename: ja5c10357_si_001-4.pdf
content_type: application/pdf
size: 5336749
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## Thumbnail
fileset_id: 936a5282-962b-4fb0-a8e8-42d52a2cac4a
filename: Xinxin Long 2025 JACS cell-surface-inter-cytochrome-electron-transfer-limits-biofilm-electron-conduction-kinetics-in-shewanella-oneidensis.pdf