# Influence of the Charge Ratio of Guanine-Quadruplex Structure-Based CpG Oligodeoxynucleotides and Cationic DOTAP Liposomes on Cytokine Induction Profiles https://mdr.nims.go.jp/datasets/2b3a1789-6f50-41e8-b5ff-8fca72765bf4 ## File - [biomolecules-13-01639.pdf](https://mdr.nims.go.jp/filesets/30bc98f2-2f27-4ff8-b2df-6dcf8b1b2b88/download) ([Detail](https://mdr.nims.go.jp/filesets/30bc98f2-2f27-4ff8-b2df-6dcf8b1b2b88.md)) - [biomolecules-2613451-supplementary.pdf](https://mdr.nims.go.jp/filesets/7ed9bbd1-2dd8-4b47-98de-98a531f50a09/download) ([Detail](https://mdr.nims.go.jp/filesets/7ed9bbd1-2dd8-4b47-98de-98a531f50a09.md)) ## Id 2b3a1789-6f50-41e8-b5ff-8fca72765bf4 ## Local identifier ## Visibility open_to_public ## State published ## Created at 2024-04-27T03:28:43.878565Z ## Updated at 2024-04-30T03:30:21.197590Z ## Published at 2024-04-30T03:30:21.768056Z ## Doi ## First published url https://doi.org/10.3390/biom13111639 ## Date published 2023-11-11 ## Recorded date published ## Resource type journal_article ## Manuscript type vor ## Collection ## Title - title: Influence of the Charge Ratio of Guanine-Quadruplex Structure-Based CpG Oligodeoxynucleotides and Cationic DOTAP Liposomes on Cytokine Induction Profiles title_type: original lang: en ## Description - description: Cationic liposomes, specifically 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) liposomes, serve as successful carriers for guanine-quadruplex (G4) structure-based cytosine-guanine oligodeoxynucleotides (CpG ODNs). The combined benefits of CpG ODNs forming a G4 structure and a non-viral vector carrier endow the ensuing complex with promising adjuvant properties. Although G4-CpG ODN-DOTAP complexes show a higher immunostimulatory effect than naked G4-CpG ODNs, the effects of the complex composition, especially charge ratios, on the production of the pro-inflammatory cytokines interleukin (IL)-6 and interferon (IFN)-alpha remain unclear. Here, we examined whether charge ratios drive the bifurcation of cytokine inductions in human peripheral blood mononuclear cells. Linear CpG ODN-DOTAP liposome complexes formed micrometer-sized positively charged agglomerates; G4-CpG ODN-DOTAP liposome complexes with low charge ratios (0.5 and 1.5) formed ~250 nm-sized negatively charged complexes. Notably, low-charge-ratio (0.5 and 1.5) complexes induced significantly higher IL-6 and IFN-alpha levels simultaneously than high-charge-ratio (2 and 2.5) complexes. Moreover, confocal microscopy indicated a positive correlation between the cellular uptake of the complex and amount of cytokine induced. The observed effects of charge ratios on complex size, surface charge, and affinity for factors that modify cellular-uptake, intracellular-activity, and cytokine-production efficiency highlight the importance of a rational complex design for delivering and controlling G4-CpG ODN activity. description_type: abstract lang: en ## Creator - name: Nguyen Bui Thao Le role: author orcid: https://orcid.org/0000-0002-4797-2339 organization: National Institute for Materials Science - name: Anh Thi Tram Tu role: author orcid: https://orcid.org/0000-0002-7243-2086 organization: National Institute for Materials Science - name: Dandan Zhao role: author organization: National Institute for Materials Science - name: Chiaki Yoshikawa role: author orcid: https://orcid.org/0000-0002-6589-387X organization: National Institute for Materials Science - name: Kohsaku Kawakami role: author orcid: https://orcid.org/0000-0002-3466-9365 organization: National Institute for Materials Science - name: Yoshihisa Kaizuka role: author orcid: https://orcid.org/0000-0002-8019-0873 organization: National Institute for Materials Science - name: Tomohiko Yamazaki role: author orcid: https://orcid.org/0000-0003-2136-8042 organization: National Institute for Materials Science ## Contact agent ## Publisher organization: MDPI AG ## Managing organization ## Keyword - subject: guanine quadruplex schema: not_defined - subject: CpG oligodeoxynucleotides schema: not_defined - subject: cationic liposomes schema: not_defined - subject: vaccine schema: not_defined - subject: immunotherapy schema: not_defined - subject: DNA delivery schema: not_defined - subject: liposome schema: not_defined ## Rights - identifier: https://creativecommons.org/licenses/by/4.0/ ## Other identifier(s) ## Data origin - data_origin_type: other ## Embargo ## Journal - title: Biomolecules issn: 2218273X volume: '13' issue: '11' article_number: '1639' ## Conference ## Related item ## Funding - identifier: 17KK0122 funder_name: Japan Society for the Promotion of Science KAKENHI description: 日本学術振興会 - identifier: TK21-017 funder_name: TIA collaborative research program “Kakehashi” description: TIA 連携プログラム探索推進事業「かけはし」 - identifier: TK22-033 funder_name: TIA collaborative research program “Kakehashi” description: TIA 連携プログラム探索推進事業「かけはし」 - identifier: 21K19057 funder_name: Japan Society for the Promotion of Science KAKENHI description: 日本学術振興会 ## Instrument ## Instrument operator ## Instrument managing organization ## Measurement method ## Specimen ## Chemical composition ## Structure for specimen ## Structural feature for specimen ## Specific property for specimen ## Process for specimen treatment ## Computational method ## Energy level/transition state ## Software ## Custom property ## Fileset - id: 30bc98f2-2f27-4ff8-b2df-6dcf8b1b2b88 filename: biomolecules-13-01639.pdf content_type: application/pdf size: 5702146 md5: 8a73aad6c0fa82d6bc23ee8a0ced921f - id: 7ed9bbd1-2dd8-4b47-98de-98a531f50a09 filename: biomolecules-2613451-supplementary.pdf content_type: application/pdf size: 1044928 md5: 9425c0cdfe6713af5ead388e6a77db47 ## Thumbnail fileset_id: 30bc98f2-2f27-4ff8-b2df-6dcf8b1b2b88 filename: biomolecules-13-01639.pdf