# Excess Ferrous Iron Promotes the Construction of Extracellular Electron Pathways in the Iron-corroding Bacterium <i>Desulfovibrio ferrophilus</i> IS5

https://mdr.nims.go.jp/datasets/284fb753-6006-429f-8156-efed0515cd7d

## File

- [Excess Ferrous Iron Promotes the Construction of Extracellular.pdf](https://mdr.nims.go.jp/filesets/11edf321-59cc-4318-abf3-cde7c0fdd599/download) ([Detail](https://mdr.nims.go.jp/filesets/11edf321-59cc-4318-abf3-cde7c0fdd599.md))

## Id

284fb753-6006-429f-8156-efed0515cd7d

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2026-04-16T02:45:08.759486Z

## Updated at

2026-04-16T06:19:59.576080Z

## Published at

2026-04-16T07:26:09.911871Z

## Doi



## First published url

https://doi.org/10.2355/isijinternational.isijint-2025-353

## Date published

2026-04-15

## Recorded date published

2026

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Excess Ferrous Iron Promotes the Construction of Extracellular Electron Pathways
    in the Iron-corroding Bacterium <i>Desulfovibrio ferrophilus</i> IS5
  title_type: original
  lang: en

## Description

- description: 'Microbial extracellular electron uptake (EEU) is central to bioelectrochemical
    processes and biocorrosion, yet its underlying mechanisms are not fully understood
    under microbially influenced iron corrosion. Here, we investigate how excess Fe2+
    modulates EEU in Desulfovibrio ferrophilus IS5, a strain that causes severe anaerobic
    iron corrosion via outer-membrane cytochromes (OMCs)-mediated electron uptake.
    We show that IS5 grown with elevated Fe2+ exhibits substantially enhanced EEU.
    This enhancement arises through two complementary mechanisms: (i) increased abundance
    of functional OMCs via the upregulation of a cytochrome assembly protein, and
    (ii) an additional electron transfer route mediated by FeS nanoparticles precipitated
    on the IS5 outer membrane. This indicates that, during iron corrosion, when IS5
    cells are found within thick layers of corrosion products and biofilms, they simultaneously
    utilize both OMCs and FeS nanoparticles to sustain high-rate EEU from iron under
    conditions of high Fe2+ concentrations and limited organic substrates. This study
    advances the mechanistic understanding of EEU-driven iron corrosion and highlights
    a potential avenue for manipulating bioelectrochemical systems.'
  description_type: abstract
  lang: und

## Creator

- name: Xiao Deng
  role: author
  orcid: https://orcid.org/0000-0002-9006-2322
  organization: National Institute for Materials Science
- name: Jun Kikkawa
  role: author
  orcid: https://orcid.org/0000-0003-0659-1844
  organization: National Institute for Materials Science
- name: Akihiro Okamoto
  role: author
  orcid: https://orcid.org/0000-0002-8102-4316
  organization: National Institute for Materials Science

## Contact agent



## Publisher

organization: Iron and Steel Institute of Japan

## Managing organization



## Keyword

- subject: microbially influenced corrosion
  schema: not_defined
- subject: sulfate-reducing bacteria
  schema: not_defined
- subject: iron sulfide nanoparticles
  schema: not_defined
- subject: outer-membrane cytochrome
  schema: not_defined
- subject: electrochemical analyses
  schema: not_defined
- subject: transmission electron microscopy
  schema: not_defined
- subject: transcriptome
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: ISIJ INTERNATIONAL
  issn: '13475460'
  volume: '66'
  issue: '5'
  start_page: 685
  end_page: 691

## Conference



## Related item



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## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Fileset

- id: 11edf321-59cc-4318-abf3-cde7c0fdd599
  filename: Excess Ferrous Iron Promotes the Construction of Extracellular.pdf
  content_type: application/pdf
  size: 856111
  md5: 9705fed8dfecaa623ffe18d117e32cf4

## Thumbnail

fileset_id: 11edf321-59cc-4318-abf3-cde7c0fdd599
filename: Excess Ferrous Iron Promotes the Construction of Extracellular.pdf