# A Novel Gene Synthesis Platform for Designing Functional Protein Polymers

https://mdr.nims.go.jp/datasets/281a42a8-c016-432c-9a70-d5085a21a53a

## File

- [25_AdvSci.pdf](https://mdr.nims.go.jp/filesets/f0e145d3-3b00-4f42-b4d4-69f46ea6df4c/download) ([Detail](https://mdr.nims.go.jp/filesets/f0e145d3-3b00-4f42-b4d4-69f46ea6df4c.md))

## Id

281a42a8-c016-432c-9a70-d5085a21a53a

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-05-31T02:02:18.341555Z

## Updated at

2026-01-06T06:59:19.494678Z

## Published at

2026-01-06T23:24:29.244791Z

## Doi



## First published url

https://doi.org/10.1002/advs.202410903

## Date published

2025-02-23

## Recorded date published

2025-4

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: A Novel Gene Synthesis Platform for Designing Functional Protein Polymers
  title_type: original
  lang: en

## Description

- description: 'In this study, a novel method is proposed herein: seamless cloning
    of rolling-circle amplicons (SCRCA). This method involves one-pot preparation
    of repetitive-sequence genes with overlapping ends for cloning, facilitating the
    easy construction of the desired recombinants. SCRCA is used to synthesize 10
    genes encoding hydrophilic resilin-like and hydrophobic elastin-like repeat units
    that induce liquid-liquid phase separation.'
  description_type: abstract
  lang: und

## Creator

- name: Toshimasa Homma
  role: author
- name: Rie Yamamoto
  role: author
- name: Lily Zuin Ping Ang
  role: author
- name: Alaa Fehaid
  role: author
  orcid: https://orcid.org/0000-0001-7759-663X
  organization: National Institute for Materials Science
- name: Mitsuhiro Ebara
  role: author
  orcid: https://orcid.org/0000-0002-7906-0350
  organization: National Institute for Materials Science

## Contact agent



## Publisher

organization: Wiley

## Managing organization



## Keyword

- subject: Gene synthesis
  schema: not_defined
- subject: Protein Polymers
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Advanced Science
  issn: '21983844'
  volume: '12'
  issue: '15'
  article_number: '2410903'

## Conference



## Related item



## Funding

- identifier: JPNP20004
  funder_name: New Energy and Industrial Technology Development Organization
- identifier: JP22680575
  funder_name: Ministry of Agriculture, Forestry and Fisheries
- identifier: AM2100
  funder_name: National Institute for Materials Science
- identifier: 22K14549
  funder_name: Japan Society for the Promotion of Science
- identifier: 24H01378
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Custom property



## Fileset

- id: f0e145d3-3b00-4f42-b4d4-69f46ea6df4c
  filename: 25_AdvSci.pdf
  content_type: application/pdf
  size: 2222778
  md5: 43edf87d3fb2cbc4436422e074761fd2

## Thumbnail

fileset_id: f0e145d3-3b00-4f42-b4d4-69f46ea6df4c
filename: 25_AdvSci.pdf