# The effect of microenvironmental viscosity on the emergence of colon cancer cell resistance to doxorubicin

https://mdr.nims.go.jp/datasets/20fccc03-2fa4-4905-ab5c-fba2c89e34a5

## File

- [Zeng-2025_The effect of microenvironmental viscosity.docx](https://mdr.nims.go.jp/filesets/f73e6281-2c43-4363-a4fc-0abac9762d79/download) ([Detail](https://mdr.nims.go.jp/filesets/f73e6281-2c43-4363-a4fc-0abac9762d79.md))

## Id

20fccc03-2fa4-4905-ab5c-fba2c89e34a5

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-07-11T00:02:48.031657Z

## Updated at

2025-07-11T04:50:38.824712Z

## Published at

2026-01-02T08:30:59.920269Z

## Doi

https://doi.org/10.48505/nims.5583

## First published url

https://doi.org/10.1039/d4tb02334j

## Date published

2025-01-02

## Recorded date published

2025-2-5

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: The effect of microenvironmental viscosity on the emergence of colon cancer
    cell resistance to doxorubicin
  title_type: original
  lang: en

## Description

- description: The colon possesses a unique physiological environment among human
    organs, where there is a highly viscous body fluid layer called the mucus layer
    above colonic epithelial cells. Dysfunction of the mucus layer not only contributes
    to the occurrence of colorectal cancer (CRC) but also plays an important role
    in the development of chemoresistance in CRC. Although viscosity is an essential
    property of the mucus layer, it remains elusive how viscosity affects chemoresistance
    in colon cancer cells. In this study, the influence of viscosity on their chemoresistance
    was elucidated by culturing colon cancer cells in media of different viscosities
    supplemented with doxorubicin (DOX). The viscosity range was adjusted from 99.4
    mPa s to 776.6 mPa s by adding polyethylene glycol of different molecular weights
    in culture medium. Cell viability in the high viscosity medium was higher than
    that in the low viscosity medium. Expression of chemoresistance-related genes
    such as ABCC2 and ABCG2 increased when cells were cultured in the high viscosity
    medium. Furthermore, cell migration increased while proliferation decreased when
    cells were cultured in the high viscosity medium. The colon cancer cells cultured
    in the high viscosity medium exhibited high expression of p21 mRNA. The results
    suggested that viscosity could affect the resistance of colon cancer cells to
    DOX by regulating the expression of chemoresistance-related and proliferation-related
    genes.
  description_type: abstract
  lang: und

## Creator

- name: Tianjiao Zeng
  role: author
  orcid: https://orcid.org/0000-0002-1286-0337
- name: Chengyu Lu
  role: author
- name: Man Wang
  role: author
- name: Huajian Chen
  role: author
  orcid: https://orcid.org/0000-0003-1643-9782
- name: Toru Yoshitomi
  role: author
  orcid: https://orcid.org/0000-0003-3847-1812
- name: Naoki Kawazoe
  role: author
  orcid: https://orcid.org/0000-0003-3916-0709
- name: Yingnan Yang
  role: author
- name: Guoping Chen
  role: author
  orcid: https://orcid.org/0000-0001-6753-3678

## Contact agent



## Publisher

organization: Royal Society of Chemistry (RSC)

## Managing organization



## Keyword

- subject: microenvironmental viscosity
  schema: not_defined
- subject: colon cancer
  schema: not_defined
- subject: resistance
  schema: not_defined
- subject: doxorubicin
  schema: not_defined

## Rights

- identifier: http://rightsstatements.org/vocab/InC/1.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2025-01-02
end_date: 2026-01-02

## Journal

- title: Journal of Materials Chemistry B
  issn: 2050750X
  volume: '13'
  issue: '6'
  start_page: 2180
  end_page: 2191

## Conference



## Related item



## Funding

- identifier: 19H04475
  funder_name: JSPS
- identifier: 24K03289
  funder_name: JSPS

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Custom property



## Fileset

- id: f73e6281-2c43-4363-a4fc-0abac9762d79
  filename: Zeng-2025_The effect of microenvironmental viscosity.docx
  content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
  size: 6641726
  md5: 15a095cfd514e2a68c9fb1228d39c28f

## Thumbnail

fileset_id: f73e6281-2c43-4363-a4fc-0abac9762d79
filename: Zeng-2025_The effect of microenvironmental viscosity.docx