# Photoactivatable substrates show diverse phenotypes of leader cells in collective migration when moving along different extracellular matrix proteins

https://mdr.nims.go.jp/datasets/1755a70f-6197-4573-bddb-36ed89e9d244

## Files

- [d4bm00225c.pdf](https://mdr.nims.go.jp/filesets/c8e0e05b-ce77-4d56-9ede-b188f75c0123/download) ([Detail](https://mdr.nims.go.jp/filesets/c8e0e05b-ce77-4d56-9ede-b188f75c0123.md))

## Id

1755a70f-6197-4573-bddb-36ed89e9d244

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-07-04T02:48:56.611191Z

## Updated at

2024-07-04T07:30:31.965974Z

## Published at

2024-07-04T07:30:32.056339Z

## Doi



## First published url

https://doi.org/10.1039/d4bm00225c

## Date published

2024-05-30

## Recorded date published

2024-6-25

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Photoactivatable substrates show diverse phenotypes of leader cells in collective
    migration when moving along different extracellular matrix proteins
  title_type: original
  lang: en

## Description

- description: In cancer metastasis, collectively migrating clusters are discriminated
    into leader and follower cells that move through extracellular matrices (ECMs)
    with different characteristics. The impact of changes in ECM protein types on
    leader cells and migrating clusters is unknown. To address this, we investigated
    the response of leader cells and migrating clusters upon moving from one ECM protein
    to another using a photoactivatable substrate bearing photocleavable PEG (PCP),
    whose surface changes from protein-repellent to protein-adhesive in response to
    light. We chose laminin and collagen I for our study since they are abundant in
    two distinct regions in living tissues, namely basement membrane and connective
    tissue. Using the photoactivatable substrates, the precise deposition of the first
    ECM protein in the irradiated areas was achieved, followed by creating well-defined
    cellular confinements. Secondary irradiation enabled the deposition of the second
    ECM protein in the new irradiated regions, resulting in region-selective heterogeneous
    and homogenous ECM protein-coated surfaces. Different tendencies in leader cell
    formation from laminin into laminin compared to those migrating from laminin into
    collagen were observed. The formation of focal adhesion and actin structures for
    cells within the same cluster in the ECM proteins responded according to the underlying
    ECM protein type. Finally, integrin β1 was crucial for the appearance of leader
    cells for clusters migrating from laminin into collagen. However, when it came
    to laminin into laminin, integrin β1 was not responsible. This highlights the
    correlation between leader cells in collective migration and the biochemical signals
    that arise from underlying extracellular matrix proteins.
  description_type: abstract
  lang: eng

## Creator

- name: Shimaa A. Abdellatef
  role: author
  organization: National Institute for Materials Science
  department: Research Center for Macromolecules and Biomaterials/Biomaterials Field/Mechanobiology
    Group
  ror: https://ror.org/026v1ze26
- name: Francesca Bard
  role: author
  organization: Cornell University
- name: Jun Nakanishi
  role: author
  orcid: https://orcid.org/0000-0003-4457-6581
  organization: National Institute for Materials Science
  department: Research Center for Macromolecules and Biomaterials/Biomaterials Field/Mechanobiology
    Group
  ror: https://ror.org/026v1ze26

## Contact agent



## Publisher

organization: Royal Society of Chemistry

## Managing organization



## Keyword

- subject: Collective cell migration
  schema: not_defined
- subject: Leader cell
  schema: not_defined
- subject: Extracellular matrix
  schema: not_defined
- subject: Photoactivatable substrate
  schema: not_defined
- subject: Mechanobiology
  schema: not_defined

## Rights

- identifier: cc-by-3.0

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Biomaterials Science
  issn: '20474830'
  volume: '12'
  issue: '13'
  start_page: 3446
  end_page: 3457

## Conference



## Related item



## Funding

- identifier: 21J40229
  funder_name: JSPS
- identifier: 22H00596
  funder_name: JSPS
- identifier: 23K17481
  funder_name: JSPS

## Instrument



## Instrument operator



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## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



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## Fileset

- id: c8e0e05b-ce77-4d56-9ede-b188f75c0123
  filename: d4bm00225c.pdf
  content_type: application/pdf
  size: 2030012
  md5: 70da89e15248f5fee828264d3750dc22

## Thumbnail

fileset_id: c8e0e05b-ce77-4d56-9ede-b188f75c0123
filename: d4bm00225c.pdf