# Oriented micropore-forming bioinks for 3D bioprinting of muscle tissues

https://mdr.nims.go.jp/datasets/14f9e602-199d-4488-b7ea-dd61d453fe6b

## File

- [Manuscript Final.pdf](https://mdr.nims.go.jp/filesets/e31740ff-9cef-4653-97d5-b15b7382fc0f/download) ([Detail](https://mdr.nims.go.jp/filesets/e31740ff-9cef-4653-97d5-b15b7382fc0f.md))

## Id

14f9e602-199d-4488-b7ea-dd61d453fe6b

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-12-05T04:26:13.658670Z

## Updated at

2026-01-06T00:18:28.710429Z

## Published at

2026-01-06T03:19:30.620961Z

## Doi

https://doi.org/10.48505/nims.6101

## First published url

https://doi.org/10.1002/smll.202509439

## Date published

2026-01-05

## Recorded date published



## Resource type

journal_article

## Manuscript type

authors_original

## Collection



## Title

- title: Oriented micropore-forming bioinks for 3D bioprinting of muscle tissues
  title_type: original
  lang: en

## Description

- description: Three-dimensional (3D) bioprinting provides a wide avenue for designing
    complex and customized constructs for regenerative medicine. Bioink formulations
    in 3D bioprinting usually lack micrometer-sized and interconnected pores for the
    supply of nutrients and oxygen and biological communications with host tissues,
    thus limiting cellular activities and therapeutic efficacy. Herein, we present
    microfibrous pore-forming bioinks for fabricating microporous hydrogels that encapsulate
    cells for muscle tissue reconstruction. Using phase separation technology, a liquid
    porogen was embedded into gelatin-based bioinks to form microfibrous structures.
    Printing bioinks with shear stress enabled the orientation of microfibrous pores
    along the printing direction, which facilitated the orientation of printed cells
    and enhanced myoblast differentiation. Moreover, the porous 3D scaffold exhibited
    promising results in terms of supplying nutrients and oxygen to improve cell survival.
    Printed tissue constructs were successfully transplanted into muscle tissue defects.
    This approach holds immense potential for creating anisotropic oriented 3D tissue
    constructs for applications in cell transplantation, drug screening, and disease
    modelling.
  description_type: abstract
  lang: eng

## Creator

- name: Debabrata Palai
  role: author
  organization: Osaka University
- name: Hana Yasue
  role: author
  organization: National Institute for Materials Science
  department: Research Center for Macromolecules and Biomaterials/Biomaterials Field/Polymeric
    Biomaterials Group
- name: Miho Ohta
  role: author
  organization: National Institute for Materials Science
  department: Research Center for Macromolecules and Biomaterials/Biomaterials Field/Polymeric
    Biomaterials Group
- name: Koichiro Uto
  role: author
  orcid: https://orcid.org/0000-0001-7091-0585
  organization: National Institute for Materials Science
  department: Research Center for Macromolecules and Biomaterials/Biomaterials Field/Smart
    Polymers Group
- name: Tetsushi Taguchi
  role: author
  orcid: https://orcid.org/0000-0003-2541-2530
  organization: National Institute for Materials Science
  department: Research Center for Macromolecules and Biomaterials/Biomaterials Field/Polymeric
    Biomaterials Group
- name: Akihiro Nishiguchi
  role: author
  orcid: https://orcid.org/0000-0002-3160-6385
  organization: National Institute for Materials Science
  department: Research Center for Macromolecules and Biomaterials/Biomaterials Field/Polymeric
    Biomaterials Group

## Contact agent



## Publisher

organization: Wiley-Blackwell

## Managing organization



## Keyword

- subject: 3D printing
  schema: not_defined
- subject: phase separation
  schema: not_defined
- subject: hydrogel
  schema: not_defined

## Rights

- description: 'This is the pre-peer reviewed version of the following article: D.
    Palai, H. Yasue, M. Ohta, K. Uto, T. Taguchi, and A. Nishiguchi, “ Oriented Micropore-Forming
    Bioinks for 3D Bioprinting of Muscle Tissues.” Small (2026): e09439, which has
    been published in final form at https://doi.org/10.1002/smll.202509439. This article
    may be used for non-commercial purposes in accordance with Wiley Terms and Conditions
    for Use of Self-Archived Versions.'
  identifier: http://rightsstatements.org/vocab/InC/1.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Small
  issn: '16136810'

## Conference



## Related item



## Funding



## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: e31740ff-9cef-4653-97d5-b15b7382fc0f
  filename: Manuscript Final.pdf
  content_type: application/pdf
  size: 1377040
  md5: 4ff116a87c494baafb8ce59a1d22ad8a

## Thumbnail

fileset_id: e31740ff-9cef-4653-97d5-b15b7382fc0f
filename: Manuscript Final.pdf