# Dynamic cell photo-manipulation technology for the molecular and mechanical regulation analyses of collective cell migration

https://mdr.nims.go.jp/datasets/1362be85-b93f-4d98-9cd3-3bb0843160a8

## File

- [1-s2.0-S266683192400033X-main.pdf](https://mdr.nims.go.jp/filesets/507ef313-7ced-4471-9ec5-ae39cedbc5b3/download) ([Detail](https://mdr.nims.go.jp/filesets/507ef313-7ced-4471-9ec5-ae39cedbc5b3.md))

## Id

1362be85-b93f-4d98-9cd3-3bb0843160a8

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-07-04T03:48:20.660467Z

## Updated at

2024-07-05T03:30:29.777229Z

## Published at

2024-07-05T03:30:29.879082Z

## Doi



## First published url

https://doi.org/10.1016/j.talo.2024.100319

## Date published

2024-04-25

## Recorded date published

2024-8

## Resource type

journal_article

## Manuscript type

vor

## Collection



## Title

- title: Dynamic cell photo-manipulation technology for the molecular and mechanical
    regulation analyses of collective cell migration
  title_type: original
  lang: en

## Description

- description: Collective cell migration is an essential biological process. Migration
    behaviors of multiple cellular units depend on the mechanical and chemical properties
    of their scaffolds and the geometry of the cells. However, the mechanisms by which
    these properties synergistically regulate the collective cell characteristics
    remain unknown. A robust method is required to analyze collective cell migration.
    Therefore, in this study, we developed a new method for collective cell migration
    analysis using defined chemical, mechanical, and geometrical properties. Our method
    is based on a poly(acrylic acid) hydrogel, whose surface is functionalized with
    photocleavable poly(ethylene glycol) and a cell-adhesive peptide. By controlling
    the UV irradiation of the photoactivatable hydrogel, we created geometrically
    controlled cellular clusters and induced collective migration. Furthermore, chemical
    and mechanical cues exposed to cell clusters were manipulated depending on the
    surface density of the cell-adhesive peptide and crosslinking density of the hydrogel.
    As a proof of concept, we also demonstrated that the collective migration of epithelial
    cells was synergistically regulated by the chemical and mechanical properties
    of the scaffold. Our results suggest the new photoactivatable substrate as a promising
    tool for advanced molecular and mechanobiological analyses of collective cell
    migration.
  description_type: abstract
  lang: und

## Creator

- name: Kazuhiro Tatematsu
  role: author
  orcid: https://orcid.org/0009-0006-1368-4925
- name: Shota Yamamoto
  role: author
  orcid: https://orcid.org/0000-0002-7422-0968
- name: Masao Kamimura
  role: author
- name: Kazuo Yamaguchi
  role: author
- name: Jun Nakanishi
  role: author
  orcid: https://orcid.org/0000-0003-4457-6581

## Contact agent



## Publisher

organization: Elsevier BV

## Managing organization



## Keyword

- subject: Collective cell migration
  schema: not_defined
- subject: Dynamic substrate
  schema: not_defined
- subject: Mechanobiology
  schema: not_defined
- subject: Extracellular Matrix
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo



## Journal

- title: Talanta Open
  issn: '26668319'
  volume: '9'
  article_number: '100319'

## Conference



## Related item



## Funding

- identifier: 22K14705
  funder_name: Japan Society for the Promotion of Science
- identifier: 22H00596
  funder_name: Japan Society for the Promotion of Science
- identifier: 23K17481
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Custom property



## Fileset

- id: 507ef313-7ced-4471-9ec5-ae39cedbc5b3
  filename: 1-s2.0-S266683192400033X-main.pdf
  content_type: application/pdf
  size: 4534785
  md5: c49685c2554e06e26815907b468fc529

## Thumbnail

fileset_id: 507ef313-7ced-4471-9ec5-ae39cedbc5b3
filename: 1-s2.0-S266683192400033X-main.pdf