Download file (4.32MB)
Download as zip (4.05MB)

コレクション

引用

説明:

Collective cell migration is an essential biological process. Migration behaviors of multiple cellular units depend on the mechanical and chemical properties of their scaffolds and the geometry of the cells. However, the mechanisms by which these properties synergistically regulate the collective cell characteristics remain unknown. A robust method is required to analyze collective cell migration. Therefore, in this study, we developed a new method for collective cell migration analysis using defined chemical, mechanical, and geometrical properties. Our method is based on a poly(acrylic acid) hydrogel, whose surface is functionalized with photocleavable poly(ethylene glycol) and a cell-adhesive peptide. By controlling the UV irradiation of the photoactivatable hydrogel, we created geometrically controlled cellular clusters and induced collective migration. Furthermore, chemical and mechanical cues exposed to cell clusters were manipulated depending on the surface density of the cell-adhesive peptide and crosslinking density of the hydrogel. As a proof of concept, we also demonstrated that the collective migration of epithelial cells was synergistically regulated by the chemical and mechanical properties of the scaffold. Our results suggest the new photoactivatable substrate as a promising tool for advanced molecular and mechanobiological analyses of collective cell migration.

権利情報:

• Creative Commons BY Attribution 4.0 International
  

キーワード: Collective cell migration, Dynamic substrate, Mechanobiology, Extracellular Matrix

刊行年月日: 2024-04-25

出版者: Elsevier BV

掲載誌:

• Talanta Open (ISSN: 26668319) vol. 9 100319

研究助成金:

• Japan Society for the Promotion of Science 22K14705
• Japan Society for the Promotion of Science 22H00596
• Japan Society for the Promotion of Science 23K17481

原稿種別: 出版者版 (Version of record)

MDR DOI:

公開URL: https://doi.org/10.1016/j.talo.2024.100319

関連資料:

その他の識別子:

連絡先:

更新時刻: 2024-07-05 12:30:29 +0900

MDRでの公開時刻: 2024-07-05 12:30:29 +0900