# Histochemical analysis of osteoclast and osteoblast distributions on hydroxyapatite/collagen bone-like nanocomposite embedded in rat tibiae

https://mdr.nims.go.jp/datasets/028f09c6-f655-4868-ae68-635463909caf

## File

- [【受理時最終版】Sakakibara_et_al_reviesed_manuscript_JOB.pdf](https://mdr.nims.go.jp/filesets/271a8d4f-2414-42b7-8356-7d7bbaed7b83/download) ([Detail](https://mdr.nims.go.jp/filesets/271a8d4f-2414-42b7-8356-7d7bbaed7b83.md))

## Id

028f09c6-f655-4868-ae68-635463909caf

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2025-01-31T08:11:58.835660Z

## Updated at

2025-02-03T01:16:01.521918Z

## Published at

2026-01-10T23:21:44.712468Z

## Doi

https://doi.org/10.48505/nims.5308

## First published url

https://doi.org/10.1016/j.job.2025.100612

## Date published

2025-01-11

## Recorded date published

2025-3

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Histochemical analysis of osteoclast and osteoblast distributions on hydroxyapatite/collagen
    bone-like nanocomposite embedded in rat tibiae
  title_type: original
  lang: en

## Description

- description: "Objectives: Hydroxyapatite (HAp)/collagen (Col) cylinders with laminated
    collagen layers were implanted into the tibial diaphysis of rats and examined
    histochemically to clarify how the orientation of HAp and Col bone-like nanocomposite
    fibers in HAp/Col blocks affects bone resorption and formation.\r\nMethods: HAp/Col
    fibers were synthesized and compressed into cylindrical blocks to mimic bone nanostructures.
    These were implanted into the cortical bone cavities of 10-week-old male Wistar
    rats with fiber bundles parallel to the tibial surface. The implants were histologically
    analyzed at 3, 5, 7, 14, and 28 days after implantation.\r\nResults: TRAP-positive
    osteoclasts appeared after 3–5 days in the lateral region of the graft, where
    the fiber ends were exposed, but not in the bottom region, where the HAp/Col fibers
    were parallel to the surface. Osteoclasts were observed in both regions by day
    14. PHOSPHO1-positive osteoblasts were first detected on day 5, appearing slightly
    away from the cylinder laterally but directly on the bottom surface. A few osteoblasts
    contacted the block laterally, whereas many were observed on the new bone tissue
    at the bottom, between days 7 and 14. Bone formation was induced earlier in the
    bottom region, whereas lateral resorption was dominant. This suggested the\r\nuncoupling
    of bone resorption and formation in the early postimplantation stages. However,
    bone remodeling shifted to coupling between osteoclasts and osteoblasts throughout
    the cylinder by day 28.\r\nConclusion: The orientation of HAp/Col fibers in HAp/Col
    graft materials substantially affected the preferential induction of bone resorption
    or formation during the early stages of bone regeneration."
  description_type: abstract
  lang: und
- description: 'これまで無配向性で作成されてきたHAp/Col緻密体やペーストなどでは骨リモデリング代謝に沿った「破骨細胞による材料吸収→骨芽細胞による骨再生」であった骨組織反応が、HAp/Colの配向を制御することで、初期に骨再生だけを誘導する面と、破骨細胞による吸収だけを誘導する面を作り出せることが明らかとなった。特に大型骨欠損を多孔体で再生させる場合、あるいは骨芽細胞を播種して再生骨とする場合の指針が明らかとなった。 '
  description_type: abstract
  lang: en

## Creator

- name: Mako Sakakibara
  role: author
- name: Tomoka Hasegawa
  role: author
  orcid: https://orcid.org/0000-0003-0309-6637
- name: Mai Haraguchi-Kitakamae
  role: author
- name: Yan Shi
  role: author
- name: Weisong Li
  role: author
- name: Jiaxin Cui
  role: author
- name: Xuanyu Liu
  role: author
- name: Tomomaya Yamamoto
  role: author
- name: Hiromi Hongo
  role: author
- name: Norio Amizuka
  role: author
- name: Yoshiaki Sato
  role: author
- name: Masanori Kikuchi
  role: author
  orcid: https://orcid.org/0000-0002-9451-8147
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26

## Contact agent



## Publisher

organization: Elsevier BV

## Managing organization



## Keyword

- subject: HAp/Col
  schema: not_defined
- subject: Bone regeneration
  schema: not_defined
- subject: Osteoblast
  schema: not_defined
- subject: Osteoclast
  schema: not_defined
- subject: Histochemistry
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2025-01-11
end_date: 2026-01-11

## Journal

- title: Journal of Oral Biosciences
  issn: '13490079'
  volume: '67'
  issue: '1'
  article_number: '100612'

## Conference



## Related item



## Funding

- identifier: 24K02609
  funder_name: Japan Society for the Promotion of Science
- identifier: 22K0991
  funder_name: Japan Society for the Promotion of Science
- identifier: 24K12864
  funder_name: Japan Society for the Promotion of Science
- identifier: 23K09115
  funder_name: Japan Society for the Promotion of Science

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



## Software



## Custom property



## Fileset

- id: 271a8d4f-2414-42b7-8356-7d7bbaed7b83
  filename: "【受理時最終版】Sakakibara_et_al_reviesed_manuscript_JOB.pdf"
  content_type: application/pdf
  size: 2101802
  md5: 3a6c45778972dd4864c48c471a183be6

## Thumbnail

fileset_id: 271a8d4f-2414-42b7-8356-7d7bbaed7b83
filename: "【受理時最終版】Sakakibara_et_al_reviesed_manuscript_JOB.pdf"