Histochemical analysis of osteoclast and osteoblast distributions on hydroxyapatite/collagen bone-like nanocomposite embedded in rat tibiae

Mako Sakakibara; Tomoka Hasegawa; Mai Haraguchi-Kitakamae; Yan Shi; Weisong Li; Jiaxin Cui; Xuanyu Liu; Tomomaya Yamamoto; Hiromi Hongo; Norio Amizuka; Yoshiaki Sato; Masanori Kikuchi

doi:10.1016/j.job.2025.100612

論文 Histochemical analysis of osteoclast and osteoblast distributions on hydroxyapatite/collagen bone-like nanocomposite embedded in rat tibiae

Mako Sakakibara ; Tomoka Hasegawa ; Mai Haraguchi-Kitakamae ; Yan Shi ; Weisong Li ; Jiaxin Cui ; Xuanyu Liu ; Tomomaya Yamamoto ; Hiromi Hongo ; Norio Amizuka ; Yoshiaki Sato ; Masanori Kikuchi (National Institute for Materials Science)

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Mako Sakakibara, Tomoka Hasegawa, Mai Haraguchi-Kitakamae, Yan Shi, Weisong Li, Jiaxin Cui, Xuanyu Liu, Tomomaya Yamamoto, Hiromi Hongo, Norio Amizuka, Yoshiaki Sato, Masanori Kikuchi. Histochemical analysis of osteoclast and osteoblast distributions on hydroxyapatite/collagen bone-like nanocomposite embedded in rat tibiae. Journal of Oral Biosciences. 2025, 67 (1), 100612. https://doi.org/10.1016/j.job.2025.100612

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(abstract)

Objectives: Hydroxyapatite (HAp)/collagen (Col) cylinders with laminated collagen layers were implanted into the tibial diaphysis of rats and examined histochemically to clarify how the orientation of HAp and Col bone-like nanocomposite fibers in HAp/Col blocks affects bone resorption and formation.
Methods: HAp/Col fibers were synthesized and compressed into cylindrical blocks to mimic bone nanostructures. These were implanted into the cortical bone cavities of 10-week-old male Wistar rats with fiber bundles parallel to the tibial surface. The implants were histologically analyzed at 3, 5, 7, 14, and 28 days after implantation.
Results: TRAP-positive osteoclasts appeared after 3–5 days in the lateral region of the graft, where the fiber ends were exposed, but not in the bottom region, where the HAp/Col fibers were parallel to the surface. Osteoclasts were observed in both regions by day 14. PHOSPHO1-positive osteoblasts were first detected on day 5, appearing slightly away from the cylinder laterally but directly on the bottom surface. A few osteoblasts contacted the block laterally, whereas many were observed on the new bone tissue at the bottom, between days 7 and 14. Bone formation was induced earlier in the bottom region, whereas lateral resorption was dominant. This suggested the
uncoupling of bone resorption and formation in the early postimplantation stages. However, bone remodeling shifted to coupling between osteoclasts and osteoblasts throughout the cylinder by day 28.
Conclusion: The orientation of HAp/Col fibers in HAp/Col graft materials substantially affected the preferential induction of bone resorption or formation during the early stages of bone regeneration.

説明:

(abstract)

これまで無配向性で作成されてきたHAp/Col緻密体やペーストなどでは骨リモデリング代謝に沿った「破骨細胞による材料吸収→骨芽細胞による骨再生」であった骨組織反応が、HAp/Colの配向を制御することで、初期に骨再生だけを誘導する面と、破骨細胞による吸収だけを誘導する面を作り出せることが明らかとなった。特に大型骨欠損を多孔体で再生させる場合、あるいは骨芽細胞を播種して再生骨とする場合の指針が明らかとなった。

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