# Injectable microcapillary network hydrogels engineered by liquid-liquid phase separation for stem cell transplantation

https://mdr.nims.go.jp/datasets/004b3b9d-c6f9-4b23-8282-3e7913c8a982

## File

- [Manuscript for Biomaterials_final version.pdf](https://mdr.nims.go.jp/filesets/89b4538e-b6a3-4a79-9668-47e3449873c6/download) ([Detail](https://mdr.nims.go.jp/filesets/89b4538e-b6a3-4a79-9668-47e3449873c6.md))

## Id

004b3b9d-c6f9-4b23-8282-3e7913c8a982

## Local identifier



## Visibility

open_to_public

## State

published

## Created at

2024-01-09T00:41:22.230006Z

## Updated at

2025-12-27T23:30:10.118843Z

## Published at

2025-12-27T23:16:37.022871Z

## Doi

https://doi.org/10.48505/nims.4692

## First published url

https://doi.org/10.1016/j.biomaterials.2023.122451

## Date published

2023-12-28

## Recorded date published

2024-3

## Resource type

journal_article

## Manuscript type

accepted_manuscript

## Collection



## Title

- title: Injectable microcapillary network hydrogels engineered by liquid-liquid phase
    separation for stem cell transplantation
  title_type: original
  lang: en

## Description

- description: Injectable hydrogels are promising carriers for cell delivery in regenerative
    medicine. However, injectable hydrogels composed of crosslinked polymer networks
    are often non-porous and prevent biological communication with host tissues through
    signals, nutrients, oxygen, and cells, thereby limiting graft survival and tissue
    integration. Here we report injectable hydrogels with liquid-liquid phase separation-induced
    microcapillary networks (µCN) as stem cell-delivering scaffolds. The molecular
    modification of gelatin with hydrogen bonding moieties induced liquid-liquid phase
    separation when mixed with unmodified gelatin to form µCN structures in the hydrogels.
    Through spatiotemporally controlled covalent crosslinking and dissolution processes,
    porous µCN structures were formed in the hydrogels, which can enhance mass transport
    and cellular activity. The encapsulation of cells with injectable µCN hydrogels
    improved cellular adhesion, spreading, migration, and proliferation. Transplantation
    of mesenchymal stem cells with injectable µCN hydrogels enhanced graft survival
    and recovered hindlimb ischemia by enhancing material-tissue communication with
    biological signals and cells through µCN. This facile approach may serve as an
    advanced scaffold for improving stem cell transplantation therapies in regenerative
    medicine.
  description_type: abstract
  lang: eng

## Creator

- name: Akihiro Nishiguchi
  role: author
  orcid: https://orcid.org/0000-0002-3160-6385
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26
- name: Shima Ito
  role: author
  orcid: https://orcid.org/0000-0002-3233-617X
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26
- name: Kazuhiro Nagasaka
  role: author
- name: Hiyori Komatsu
  role: author
  orcid: https://orcid.org/0000-0002-2525-1362
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26
- name: Koichiro Uto
  role: author
  orcid: https://orcid.org/0000-0001-7091-0585
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26
- name: Tetsushi Taguchi
  role: author
  orcid: https://orcid.org/0000-0003-2541-2530
  organization: National Institute for Materials Science
  ror: https://ror.org/026v1ze26

## Contact agent



## Publisher

organization: Elsevier BV

## Managing organization



## Keyword

- subject: Regenerative medicine, Hydrogel, Liquid-liquid phase separation, Porous
    material, Mechanobiology
  schema: not_defined

## Rights

- identifier: https://creativecommons.org/licenses/by-nc-nd/4.0/

## Other identifier(s)



## Data origin

- data_origin_type: other

## Embargo

start_date: 2023-12-28
end_date: 2025-12-28

## Journal

- title: Biomaterials
  issn: '01429612'
  volume: '305'
  article_number: '122451'

## Conference



## Related item



## Funding

- identifier: 20K20207
  funder_name: Japan Society for the Promotion of Science
- identifier: 22H03962
  funder_name: Japan Society for the Promotion of Science
- identifier: 23H01718
  funder_name: Japan Society for the Promotion of Science
- funder_name: Uehara Memorial Foundation

## Instrument



## Instrument operator



## Instrument managing organization



## Measurement method



## Specimen



## Chemical composition



## Structure for specimen



## Structural feature for specimen



## Specific property for specimen



## Process for specimen treatment



## Computational method



## Energy level/transition state



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## Fileset

- id: 89b4538e-b6a3-4a79-9668-47e3449873c6
  filename: Manuscript for Biomaterials_final version.pdf
  content_type: application/pdf
  size: 2208966
  md5: 3be92b6371bf9911ca13b7cc0db505ff

## Thumbnail

fileset_id: 89b4538e-b6a3-4a79-9668-47e3449873c6
filename: Manuscript for Biomaterials_final version.pdf